Study for pathogenesis of Moyamoya disease using induced pluripotent stem cells

• Project/Area Number: 25462222
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Saga University
• Principal Investigator: Matsuo Muneaki 佐賀大学, 医学部, 教授 (20219398)
• Co-Investigator(Kenkyū-buntansha): 江良 択実 熊本大学, 発生医学研究所, 教授 (00273706)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 脳血管障害 / 人工多能性幹細胞 / 細胞外マトリックス / 血流ストレス / 脳神経疾患 / 血管内皮細胞 / 多機能幹細胞 / もやもや病 / iPS細胞

Outline of Final Research Achievements

We analyzed the autopsy specimen of moyamoya disease by using immunohistochemistry methods. The surface matrix of moyamoya disease patient contains less amount of hyaruronan and chondroitin sulfate, thus the endothelial cells of moyamoya disease are vulnerable to shear stress of blood stream. As a result, repair cells are invade into the vascular wall and produce excessive amount of hyarulonan, then cause vascular occulusion.