Intra-arterial transplantation of low-dose stem cells provides functional recovery without adverse effects after stroke.
| Project/Area Number |
25462223
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
FUKUDA Yutaka 長崎大学, 医歯薬学総合研究科(医学系), 客員研究員 (20646575)
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| Co-Investigator(Kenkyū-buntansha) |
HORIE Nobutaka 長崎大学, 病院(医学系), 助教 (70380912)
SATO Katsuya 長崎大学, 医歯薬学総合研究科(医学系), 教授 (70398147)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 脳梗塞 / 再生医療 / 幹細胞 / 移植 |
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| Outline of Final Research Achievements |
At 24 hours after middle cerebral artery occlusion and reperfusion, we administered human mesenchymal stem cells (MSCs) (low dose: 1 × 104 cells; high dose: 1 × 106 cells) and then assessed functional recovery, inflammatory responses, cell distribution, and mortality. Rats treated with high- or low-dose MSCs showed behavioral recovery. At day 8 post-stroke, microglia activation was suppressed significantly, and interleukin (IL)-1β and IL-12p70 were reduced in both groups. Although high-dose MSCs were more widely distributed in the cortex and striatum of rats, the degree of intravascular cell aggregation and mortality was significantly higher in the high-dose group. In conclusion, selective intra-arterial transplantation of low-dose MSCs has anti-inflammatory effects and reduces the adverse effects of embolic complication, resulting in sufficient functional recovery of the affected brain.
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Report
(3 results)
Research Products
(6 results)
