Suicide gene theraphy for malignant glioma using induced pluripotent stem cells

Research Project

Project/Area Number	25462252
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Neurosurgery
Research Institution	Hamamatsu University School of Medicine
Principal Investigator	Amano Shinji 浜松医科大学, 医学部, 特任研究員 (70464138)
Co-Investigator(Kenkyū-buntansha)	Namba Hiroki 浜松医科大学, 医学部, 教授 (60198405)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	自殺遺伝子療法 / iPS細胞 / bystander効果 / mesenchymal stem cell
Outline of Final Research Achievements	Suicide gene theraphy for malignant glioma using induced pluripotent stem cells(iPS cells) was studied. Mouse iPS cells indicated accumulation in a various glioma cell lines. The bystander effect with the glioma cells didn't give us the good effect, because cell contact is obstructed by speed of the iPS cell division and the colony formation. When I made a mouse iPS cell differentiate into mesenchymal stem cell (MSC), and it was considered, good anti-tumor effect was admitted. The MELK gene which participates in colony formation was restrained in SiomycinA, but the effect was impartial. MELK genetic restraint could see reinforcement of the effect to cancer stem cell.