| Project/Area Number |
25462266
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Ehime University |
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Principal Investigator |
Onishi Takanori 愛媛大学, 医学(系)研究科(研究院), 教授 (70233210)
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| Co-Investigator(Kenkyū-buntansha) |
Oue Shiro 愛媛大学, 大学院医学系研究科, 准教授 (70213626)
Kono Shohei 愛媛大学, 医学部附属病院, 講師 (70467851)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 悪性グリオーマ / 浸潤 / 癌幹細胞 / microRNA / グリオーマ幹細胞 / 腫瘍増殖 / 腫瘍浸潤 / PLAT / グリオーマ / 腫瘍幹細胞 / 腫瘍発生 |
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| Outline of Final Research Achievements |
Glioma-initiating cells(GICs) were obtained from the primary culture of human glioma tissues and they were presented for a comprehensive analysis of microRNA(miR) by the miR microarrays. We identified a novel miR-340 that was aberrantly down-regulated in all GICs. To examine the functions of miR-340, miR-340 gene was overexpressed in GICs. The miR-340 overexpressed GICs significantly decreased cell proliferation, invasion, migration in vitro and the GICs transplanted in the mouse brain could not generate a tumor. In addition, we defined PLAT(TPA: tissue plasminogen activator) as the direct target gene for miR-340. Knockdown of PLAT with the shRNA also inhibited cell proliferation, invasion, migration and tumorigenesis in mouse brains. These results indicate that miR-340 can play a role as a tumor suppressor in glioma stem cells through the target gene, PLAT
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