Identification of novel prognostic markers for glioblastomas by means of next-generation sequencing-ased targeted resequencing
| Project/Area Number |
25462283
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | National Cancer Center Japan (2015)
National Center of Neurology and Psychiatry (2013) |
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Principal Investigator |
ICHIMURA Koichi 国立研究開発法人国立がん研究センター, その他部局等, 分野長 (40231146)
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| Co-Investigator(Renkei-kenkyūsha) |
USHIJIMA Toshikazu 国立がん研究センター, エピゲノム解析分野, 分野長 (90232818)
NARITA Yoshitaka 国立がん研究センター, 脳脊髄腫瘍科, 科長 (40392344)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 脳腫瘍 / 遺伝子変異 / 次世代シークエンス / 膠芽腫 / 分子分類 / TERT / グリオーマ / Ion Torrent / 遺伝子診断 / マイクロアレイ / CGH / IonTorrent |
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| Outline of Final Research Achievements |
Glioblastomas are the most common and malignant brain tumors. For molecular classification of gliomas, we established a series of robust assays to screen IDH mutations, TERT promoter mutations, 1p/19q codeletion and MGMT methylation. Using these assays, a total of 951 adult gliomas including 453 glioblastomas collected through a multi-center collaboration were studied. We found that a molecular classification by a combination of IDH mutation and TERT mutation was strongly associated with prognosis of the glioma patients. In addition, we found that a significant interaction between TERT mutation and MGMT methylation was associated with prognosis of glioblastomas and that the combination of these alterations could serve as a useful prognostic marker. We have also established a Glioma Mutation Screening Panel for 93 genes known to be frequently mutated in gliomas. Using this panel, we are screening glioblastomas for mutations to identify a novel prognostic marker(s).
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Glioblastomas with IDH1/2 mutations have a short clinical history and have a favourable clinical outcome2016
Author(s)
Ohno M, Narita Y, Miyakita Y, Matsushita Y, Arita H, Yonezawa M, Yoshida A, Fukushima S, Takami H, Ichimura K, Shibui S
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Journal Title
Japanese Journal of Clinical Oncology
Volume: 46
Issue: 1
Pages: 31-39
DOI
Related Report
Peer Reviewed
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[Journal Article] Upregulating mutations in the TERT promoter commonly occur in adult malignant gliomas and are strongly associated with total 1p19q loss.2013
Author(s)
Arita H, Narita Y, Fukushima S, Tateishi K, Matsushita Y, Yoshida A, Miyakita Y, Ohno M, Collins VP, Kawahara N, Shibui S, Ichimura K.
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Journal Title
Acta Neuropathol.
Volume: 126
Issue: 2
Pages: 267-276
DOI
Related Report
Peer Reviewed
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