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The basic reaserch for the pathologic symptom to improve the survival rate of transplanted bone marrow stromal cells in the injured spinal cord

Research Project

Project/Area Number 25462290
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionUniversity of Fukui

Principal Investigator

NAKAJIMA Hideaki  福井大学, 医学部, 助教 (10397276)

Co-Investigator(Kenkyū-buntansha) UCHIDA Kenzo  福井大学, 医学部, 教授 (60273009)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords脊髄損傷 / 骨髄間質細胞 / 細胞移植 / 脊髄損傷後疼痛 / 神経障害性疼痛 / 抗IL-6受容体抗体
Outline of Final Research Achievements

One of the problems of bone marrow stromal cells (BMSC) transplantation for spinal cord injury (SCI) is the low survival rate in the injured spinal cord. Some research indicated that the survival rate of transplanted BMSC are correlated with the functional recovery.
A temporal blockade of IL-6 signaling after SCI abrogates damaging inflammatory activity, especially MAPK pathway, and promotes functional recovery by promoting the survival rate of transplanted BMSC. The mechanism of transplanted BMSC death is apoptosis (Annexin-V) but not necrosis (PI-positive) by flow cytometry.
BMSC transplantation at day 3 post-SCI improved motor function and relieved SCI-induced hypersensitivities to mechanical and thermal stimulation through a variety of related mechanisms that include neuronal sparing and restoration of the disturbed blood-spinal cord barrier, mediated through modulation of the activity of spinal-resident microglia and the activity and recruitment of hematogenous macrophages.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (6 results) Book (1 results)

  • [Journal Article] Early Transplantation of Mesenchymal Stem Cells after Spinal Cord Injury Relieves Pain Hypersensitivity Through Suppression of Pain‐ Related Signaling Cascades and Reduced Inflammatory Cell Recruitment2015

    • Author(s)
      S Watanabe, K Uchida, H Nakajima, H Matsuo, D Sugita, A Yoshida, K Honjoh, Johnson WE, H Baba
    • Journal Title

      Stem Cells

      Volume: in press Issue: 6 Pages: 1902-1914

    • DOI

      10.1002/stem.2006

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 脊髄損傷に対する間葉系幹細胞移植後の脊髄再生2015

    • Author(s)
      内田研造、中嶋秀明、渡邉修司、髙橋藍、本定和也
    • Organizer
      第30回日本整形外科学会基礎学術集会
    • Place of Presentation
      富山
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脊髄損傷部および腰膨大部におけるhematogenous macrophageおよびresident microgliaの動態2015

    • Author(s)
      中嶋秀明、内田研造、渡邉修司、本定和也
    • Organizer
      第44回日本脊椎脊髄病学会
    • Place of Presentation
      福岡
    • Year and Date
      2015-04-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 抗IL-6受容体抗体が脊髄損傷後移植骨髄間質細胞の生存率に与える影響2013

    • Author(s)
      中嶋秀明、内田研造、渡邉修司、Tan Ying、Alexander Rodriguez Guerrero、馬場久敏
    • Organizer
      第42回脊椎脊髄病学会
    • Place of Presentation
      沖縄(沖縄コンベンションセンター)
    • Related Report
      2013 Research-status Report
  • [Presentation] 抗IL-6抗体投与が移植骨髄間質細胞の生存率に与える影響2013

    • Author(s)
      中嶋秀明、内田研造、渡邉修司、Tan Ying、Alexander Rodriguez Guerrero、馬場久敏
    • Organizer
      第86回日本整形外科学会
    • Place of Presentation
      広島(リーガロイヤルホテル広島)
    • Related Report
      2013 Research-status Report
  • [Presentation] 損傷脊髄内移植骨髄間質細胞の細胞死メカニズムと抗IL-6受容体抗体併用による移植効率改善効果2013

    • Author(s)
      中嶋秀明、内田研造、渡邉修司、Alexander Rodriguez Guerrero、本定和也、馬場久敏
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      千葉(幕張メッセ)
    • Related Report
      2013 Research-status Report
  • [Presentation] 脊髄損傷急性期における抗IL-6受容体抗体投与によるmacrophage極性変化と機能改善効果2013

    • Author(s)
      中嶋秀明、内田研造、Alexander Guerrero、渡邉修司、馬場久敏
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      千葉(幕張メッセ)
    • Related Report
      2013 Research-status Report
  • [Book] Neuroprotection and Regeneration of the Spinal Cord2014

    • Author(s)
      Nakajima H, Uchida K, Guerrero AR, Watanabe S, Baba H.
    • Total Pages
      419
    • Publisher
      Springer
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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