Evaluation of neuronal tract in SBA using two-photon excited microscopy

• Project/Area Number: 25462295
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Ehime University
• Principal Investigator: Matsuda Seiji 愛媛大学, 医学(系)研究科(研究院), 教授 (40173843)
• Co-Investigator(Renkei-kenkyūsha): Hamada Fumihiko 大分大学, 医学系研究科, 教授 (70252707)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 二分脊椎 / ヒヨコ / 神経障害 / GABA / カスパーゼ / 神経伝導路 / 二光子顕微鏡 / 神経回路 / ニワトリ / 歩行障害

Outline of Final Research Achievements

Spina bifida aperta (SBA) is a complex congenital malformation of spinal cord. Since GABA neurons play vital roles in spinal injury-induced neurological disorders, their roles are postulated in the pathogenesis of SBA. 1) We found higher levels of GABA neurons in the lumber cord of SBA chicks than normal chicks at embryonic days 14 and 18, while drastic reduction of them especially around motor neurons were observed in SBA chicks at post-hatch day 4. Reduction of GABA neurons was coincided with severe neurological dysfunctions, leg movement disorders and painful states of SBA chicks. 2) An increased activation of caspase 3, a marker of apoptosis, was also observed in lumber cord of SBA chicks. Taken together, it seems that increased neurological dysfunctions, loss of GABA neurons, and increased activity of caspase 3 strongly suggests a causal link among these processes. 3)Injured axons were evaluated using two-photon excited fluorescence microscopy after spinal cord contusion injury.

Research Products

• [Journal Article] 18-mer Peptide Derived from Prosaposin ameliorates the effect of Aβ1-42 neurotoxicity on hippocampal neurogenesis and memory deficit (2016)
  • Author(s): Hui-ling Gao, Cheng Li, Hiroaki Nabeka, Tetsuya Shimokawa, Zhan-You Wang, Ya-ming Cao, Seiji Matsuda
  • Journal Title: J Alzheimer disease Volume: in press
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evaluation of Injured Axons Using Two-Photon Excited Fluorescence Microscopy after Spinal Cord Contusion Injury in YFP-H Line Mice (2015)
  • Author(s): Horiuchi H, Oshima Y, Ogata T, Morino T, Matsuda S, Miura H, Imamura T
  • Journal Title: International Journal of Molecular Sciences Volume: 16(7) Issue: 7 Pages: 15785-99
  • DOI: 10.3390/ijms160715785
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ontogeny of corticotrophin-releasing hormone system in slow- and fast-growing chicks (Gallus gallus) (2015)
  • Author(s): Md. Sakirul Islam Khan, Chisa Shigeoka, Yuriko Takahara, Seiji Matsuda, Tetsuya Tachibana
  • Journal Title: Physiology and Behavio Volume: 151 Pages: 38-45
  • Peer Reviewed
• [Journal Article] Ontogenetic shape change in the chicken brain: implications for paleontology (2015)
  • Author(s): Kawabe S,Matsuda S, Tsunekaw Na, Endo H
  • Journal Title: PLOS ONE Volume: 10 Pages: 1-16
  • Peer Reviewed / Open Access
• [Journal Article] A prosaposin-derived peptide alleviates kainic acid-induced brain injury (2015)
  • Author(s): Nabeka H, Shiomokawa T, Doihara T, Saito S, Wakisaka H, Hamada F, Kobayashi N,Matsuda S
  • Journal Title: PLOS ONE Volume: 10 Pages: 1-18
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Differential expression of the alternatively spliced forms of prosaposin mRNAs in the rat choroid plexus. (2014)
  • Author(s): Saito S., Saito K., Nabeka H., Shimokawa T., Kobayashi N., Marsuda S.
  • Journal Title: Cell Tissue Res. Volume: 356 Issue: 1 Pages: 231-242
  • DOI: 10.1007/s00441-013-1773-9
  • Peer Reviewed
• [Journal Article] The nicotinic cholinergic system is affected in rats with delayed carbon monoxide encephalopathy (2014)
  • Author(s): S Ochi, M Abe, C Li, Y MORI, T Ishimaru, Y Yoshino, K Yamazaki, T Mori, R Fukuhara, S Tanimukai, S Matuda, K Yoshida, S Ueno
  • Journal Title: Neuroscience Letter Volume: IN PRESS Pages: 33-37
  • DOI: 10.1016/j.neulet.2014.03.054
  • Peer Reviewed
• [Journal Article] Temporal Changes in Prosaposin Expression in the Rat Dentate Gyrus after Birth (2014)
  • Author(s): M Morishita, H Nabeka, T Shimokawa, K Miyawaki, T Doihara, S Saito, N Kobayashi, S Matsuda
  • Journal Title: PLOS ONE Volume: 9(5)
  • Peer Reviewed
• [Journal Article] Elimination and active extrusion of liver mitochondrial proteins during lipopolysaccharide administration in rat (2013)
  • Author(s): Kana Unuma, Toshihiko Aki, Seiji Matsuda, Takeshi Funakoshi, Ken-ichi Yoshida, Koichi Uemura
  • Journal Title: Hepatology Research Volume: 43 Pages: 526-534
  • NAID: 10031183486
  • Peer Reviewed
• [Journal Article] Inducer of heme oxygenase-1 cobalt protoporphyrin accelerates autophagy and suppresses oxidative damages during lipopolysaccharide treatment in rat liver. (2013)
  • Author(s): Kana Unuma1, Toshihiko Aki, Seiji Matsuda, Takeshi Funakoshi1, Ken-ichi Yoshida3, Koichi Uemura
  • Journal Title: Hepatology Research Volume: 43 Pages: 91-96
  • NAID: 10031183462
  • Peer Reviewed
• [Journal Article] Prosaposin expression in the regenerated muscles of mdx and cardiotoxin-treated mice (2013)
  • Author(s): Cheng Li, Hui-ling Gao, Tetsuya Shimokawa, Hiroaki Nabeka, Fumihiko Hamada, Hiroaki Araki, Ya-ming Cao, Naoto Kobayashi, Seiji Matsuda
  • Journal Title: Hisotology and Histopathology Volume: 28 Pages: 875-892
  • Peer Reviewed
• [Journal Article] Attenuation of MPTP/MPP+ Toxicity in vivo and in vitro by an 18-mer Peptide Derived from Prosaposin (2013)
  • Author(s): H Gao, C Li, H Nabeka, T Shimokawa, S Saito, ZY Wang, Y Cao, S Matsuda
  • Journal Title: Neuroscience Volume: 236 Pages: 373-393
  • Peer Reviewed
• [Journal Article] Relationship between brain volume and brain width in mammals and birds (2013)
  • Author(s): S Kawabe, T Shimokawa, H Miki, T Okamoto, S Matsuda, T Itou, H Koie, M Kitagawa, T Sakai, M Hosojima, H Endo
  • Journal Title: Paleontological Research Volume: 17 Pages: 282-293
  • NAID: 10031178444
  • Peer Reviewed
• [Journal Article] Distribution of prosaposin in rat lymphatic tissues (2013)
  • Author(s): T Shimokawa, H Nabeka, K Yamamiya, H Wakisaka, T Takeuchi, N Kobayashi, S Matsuda
  • Journal Title: Cell Tissue Res Volume: 352 Pages: 685-693
  • Peer Reviewed
• [Journal Article] Variation in avian brain shape: relationship with size and orbital shape (2013)
  • Author(s): S Kawabe, T Shimokawa, H Miki, S Matsuda, H Endo
  • Journal Title: J Anatomy Volume: 223(5) Issue: 5 Pages: 495-508
  • DOI: 10.1111/joa.12109
  • Peer Reviewed
• [Journal Article] Decrease in prosaposin in the dystrophic mdx mouse brain (2013)
  • Author(s): Hl Gao, C Li, H Nabeka, T Shimokawa, N Kobayashi, S Saito, ZY Wang, YM Cao, S Matsuda
  • Journal Title: PLOS ONE Volume: 8(11)
  • Peer Reviewed
• [Presentation] Loss of GABAergic neurons coincide with motor dysfunctions in a chick model of spina bifida aperta. (2016)
  • Author(s): Khan MSI, Islam F, Xuan L, 下川哲哉、 鍋加浩明、 土居原拓也、山宮公子、 松田正司
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: 福島県、郡山市、ビッグパレットふくしま
  • Year and Date: 2016-03-28
• [Presentation] 手術的二分脊椎モデルにおける脊髄前角細胞の解析及び運動機能の異常について (2014)
  • Author(s): 三好翔太、 鍋加 浩明 , 下川 哲哉 ,土居原 拓也, 小林 直人, 松田 正司
  • Organizer: 解剖学会全国集会
  • Place of Presentation: 栃木、自治医科大学
• [Book] Neural Tube Defects: Prevalence, Pathogenesis and Prevention, NOVA Science publisher (2013)
  • Author(s): S Matsuda, H Nabeka, T Shimokawa
  • Publisher: NOVA Science publisher