| Project/Area Number |
25462297
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
Yone Kazunori 鹿児島大学, 医歯学域医学系, 教授 (40182844)
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| Co-Investigator(Kenkyū-buntansha) |
Harutoshi Sakakima 鹿児島大学, 医歯学域医学系, 教授 (10325780)
Fumiyo Matsuda 鹿児島大学, 医歯学域医学系, 助教 (70437953)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 脊髄損傷 / Stem Cell factor / 二次損傷 / stem cell factor / 一次損傷 / 急性脊髄損傷 / 脊髄変性 / Stem Cell Factor / アポトーシス / Bcl-2 |
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| Outline of Final Research Achievements |
The purpose of this study is to examine the mechanism of preventive effect of Stem cell factor (SCF) on neural cells apoptosis after acute spinal cord injury. Forty Wister male rats were randomly divided four groups as follows, sham group, SCF group, control group and HA+SCF group. After anesthesia, the spinal cord was injured by applying 120g extradural static weight-compression for two minutes using a cylindrical compressor to make permanent complete spinal cord injury. The drugs were administered by intraperitoneal injection fifteen minutes after surgery. The spinal cord were taken at twenty four and seventy two hours after surgery. The tissues were stained for Hematoxylin and Eosin, Nissle, and Luxol fast blue for histological observation after spinal cord injury. In control group, large hematomas in the dorsal horn were formed and the number of nuclei was decreased. But hematomas formation and the number of nuclei were less apparent in SCF group.
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