| Project/Area Number |
25462301
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka City University |
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Principal Investigator |
Dohzono Sho 大阪市立大学, 大学院医学研究科, 登録医 (10618945)
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| Co-Investigator(Kenkyū-buntansha) |
TERAI Hidetomi 大阪市立大学, 大学院医学研究科, 准教授 (20382046)
TOYODA Hiromitsu 大阪市立大学, 大学院医学研究科, 講師 (50514238)
NAKAMURA Hiroaki 大阪市立大学, 大学院医学研究科, 教授 (60227931)
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| Research Collaborator |
TSUKIYAMA Kuniaki
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 黄色靭帯肥厚 / 機械的ストレス / 腰部脊柱管狭窄 / ウサギ / PCR / real-time PCR |
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| Outline of Final Research Achievements |
The aims of this research were ① to prove that yellow ligament thickening model in rabbit is useful, and ② to assess the changes in the thickened yellow ligament histologically . With regard to ①, L2/3 and L4/5 were fixed with plates and harvested 8 and 16 weeks after surgery. We found that range of motion at L3/4 increased. With regard to ②, we found that toluidine blue staining showed red-purple areas, which are known to represent metachromasia indicating cartilagespecific matrix, were prominently increased in the yellow ligament. Moreover, these cells and the matrix were positively stained with an antitype 2 collagen antibody by immunohistochemistry.
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