| Project/Area Number |
25462303
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
Tsutsui Shunji 和歌山県立医科大学, 医学部, 講師 (70423960)
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| Co-Investigator(Kenkyū-buntansha) |
Taniguchi Wataru 和歌山県立医科大学, 医学部, 助教 (20453194)
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| Co-Investigator(Renkei-kenkyūsha) |
Sonekatsu Mayumi 和歌山県立医科大学, 医学部, 学内助教 (40725579)
Nishio Naoko 和歌山県立医科大学, 医学部, 特別研究員 (40648359)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 顆粒球コロニー刺激因子 / G-CSF / パッチクランプ法 / in vivoパッチクランプ法 / 脊髄後角 / シグナル伝達 / 興奮性シナプス後電流 / 抑制性シナプス後電流 / パッチクランプ |
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| Outline of Final Research Achievements |
We investigated effects of Granulocyte colony-stimulating factor (G-CSF) onto the synaptic transmission of the rat spinal dorsal horn neurons by patch-clamp methods. In our study, bath application of G-CSF tended to enhance spontaneous excitatory postsynaptic currents (sEPSCs) of dorsal horn neurons. On the other hand, G-CSF had no effect onto spontaneous inhibitory postsynaptic currents (sIPSCs) of dorsal horn neurons. Moreover, we analyzed how G-CSF affected dorsal horn neurons by in vivo patch-clamp. It showed that sEPSCs were slightly inhibited by bath application of G-CSF. These results suggest that dorsal horn neurons have cell-specific response to G-CSF. We think that inhibitory effects onto excitatory neurotransmission by G-CSF lead analgesic actions.
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