Effect of amiloride on endoplasmic reticulum stress response in the injured spinal cord of rats
| Project/Area Number |
25462311
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUYAMA Kaori 東海大学, 医学部, 助教 (20433914)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 脊髄損傷 / 小胞体ストレス / オリゴデンドロサイト前駆細胞 / アミロライド / オリゴデンドロサイト / インフラマソーム / 小胞体ストレス応答 / アポトーシス / ２次損傷 |
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| Outline of Final Research Achievements |
After spinal cord injury, secondary injury results in an expanding area of glial cell apoptosis. Oligodendrocyte precursor cells (OPC), that are involved in remyelination, have been shown to actively proliferate, but a majority of these cells succumb to apoptosis instead of differentiating into functional oligodendrocytes. In this study, We focused on endoplasmic reticulum (ER) stress as a cause of apoptosis of oligodendrocyte precursor cell following spinal cord injury, and examined it in vivo. We labeled proliferating OPCs and demonstrated that amiloride treatment led to greater numbers of OPCs and also oligodendrocytes in the injured spinal cord. The increased MBP expression suggests that the increased numbers of mature oligodendrocytes led to improved remyelination, which significantly improved motor function recovery.
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Report
(3 results)
Research Products
(13 results)
