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Effect of amiloride on endoplasmic reticulum stress response in the injured spinal cord of rats

Research Project

Project/Area Number 25462311
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionTokai University

Principal Investigator

WATANABE Masahiko  東海大学, 医学部, 教授 (40220925)

Co-Investigator(Kenkyū-buntansha) SUYAMA Kaori  東海大学, 医学部, 助教 (20433914)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords脊髄損傷 / 小胞体ストレス / オリゴデンドロサイト前駆細胞 / アミロライド / オリゴデンドロサイト / インフラマソーム / 小胞体ストレス応答 / アポトーシス / 2次損傷
Outline of Final Research Achievements

After spinal cord injury, secondary injury results in an expanding area of glial cell apoptosis. Oligodendrocyte precursor cells (OPC), that are involved in remyelination, have been shown to actively proliferate, but a majority of these cells succumb to apoptosis instead of differentiating into functional oligodendrocytes. In this study, We focused on endoplasmic reticulum (ER) stress as a cause of apoptosis of oligodendrocyte precursor cell following spinal cord injury, and examined it in vivo. We labeled proliferating OPCs and demonstrated that amiloride treatment led to greater numbers of OPCs and also oligodendrocytes in the injured spinal cord. The increased MBP expression suggests that the increased numbers of mature oligodendrocytes led to improved remyelination, which significantly improved motor function recovery.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Endoplasmic reticulum stress response in the rat contusive spinal cord injury model―susceptibility in specific cell types2014

    • Author(s)
      Matsuyama D, Watanabe M, Suyama K, Kuroiwa M, Mochida J
    • Journal Title

      Spinal Cord

      Volume: 52 Issue: 1 Pages: 9-16

    • DOI

      10.1038/sc.2013.118

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] GRP78 suppresses lipid peroxidation and promotes cellular antioxidant levels in glial cells following hydrogen peroxide exposure.2014

    • Author(s)
      Suyama K, Watanabe M, Sakabe K, Otomo A, Okada Y, Terayama H, Imai T, Mochida J.
    • Journal Title

      PLoS One

      Volume: 24;9(1) Issue: 1 Pages: e86951-e86951

    • DOI

      10.1371/journal.pone.0086951

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Amiloride Promotes Oligodendrocyte Survival And Remyelination After Spinal Cord Injury In Rat2016

    • Author(s)
      Imai T, Watnabe M, Katoh H,Yanagisawa S, Suyama K, Kuriowa M,Mochida J
    • Organizer
      Orthopaedic Research Society 2016 Annual Meeting
    • Place of Presentation
      アメリカ オーランド ホテルディズニーコロナドスプリングリゾート
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 脊髄損傷ラットにおけるアミロライド投与によるオリゴデンドロサイトの生存および髄鞘形成への影響2015

    • Author(s)
      今井洸 渡辺雅彦 加藤裕幸 持田讓治
    • Organizer
      第30回日本整形外科学会基礎学術集会
    • Place of Presentation
      富山市 富山国際会議場
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Spinal Cord Injury Current and Future Treatment Strategies2015

    • Author(s)
      Watanabe M, Mochida J
    • Organizer
      Spine Across the Sea 2015
    • Place of Presentation
      アメリカ ハワイ ホテルフェアモント オーキッド
    • Year and Date
      2015-07-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Amiloride promotes oligodendrocyte survival and remyelination after spinal cord injury in rats2015

    • Author(s)
      Watanabe M, Katoh H, Imai T,Matsuyama D, Kuroiwa M, Mochida J
    • Organizer
      The 4th ISCOS & ASIA joint scientific meeting
    • Place of Presentation
      カナダ モントリオール ホテルクイーンエリザベス2
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Grp78 suppresses lipid peroxidation of glial cell membrane by oxidative stress in traumatic spinal cord injury2014

    • Author(s)
      Suyama K, Watanabe M, Sakabe K, Otomo A, Okada Y, Terayama H, Imai T,Mochida J
    • Organizer
      60th Annual Meeting of Orthopaedic Research Society
    • Place of Presentation
      New Orleans
    • Related Report
      2013 Research-status Report
  • [Presentation] グリア細胞における小胞体シャペロンGRP78の抗酸化因子制御効果2014

    • Author(s)
      隅山香織、渡辺雅彦、 寺山隼人、 持田讓治、 坂部貢
    • Organizer
      第119回日本解剖学会総会・全国学術集会
    • Place of Presentation
      栃木県下野市
    • Related Report
      2013 Research-status Report
  • [Presentation] Effect of amiloride to endplasmic reticulum stress response in injured spinal cord.2013

    • Author(s)
      Kuroiwa M, Watanabe M, Katoh H, Imai T, Suyama K, Matsuyama D, Mochida J
    • Organizer
      Society for Neuroscience 2013 annual meeting
    • Place of Presentation
      San Diego
    • Related Report
      2013 Research-status Report
  • [Presentation] 損傷脊髄における小胞体ストレスに対するアミロライドの効果2013

    • Author(s)
      黒岩真弘、渡辺雅彦、加藤裕幸、 隅山香織、 松山大輔、 今井洸、 持田讓治
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      幕張
    • Related Report
      2013 Research-status Report
  • [Presentation] グリア細胞における抗酸化因子制御に対する小胞体シャペロンGRP78の関与2013

    • Author(s)
      隅山香織、 渡辺雅彦、 坂部貢、 持田讓治
    • Organizer
      第48回日本脊髄障害医学会
    • Place of Presentation
      福岡
    • Related Report
      2013 Research-status Report
  • [Presentation] 抗酸化因子グルタチオンを介した小胞体ストレス応答による脊髄損傷における細胞膜脂質過酸化の抑制2013

    • Author(s)
      隅山香織、渡辺雅彦、 坂部貢、 岡田義則、 黒岩真弘、 持田讓治
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      幕張
    • Related Report
      2013 Research-status Report
  • [Book] Neuroprotection and Regeneration of the Spinal Cord2014

    • Author(s)
      Watanabe M, Katoh H, Kuriowa M, Imai T, Suyama K, Matsuyama D, Mochida J
    • Publisher
      Springer
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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