| Project/Area Number |
25462314
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Doshisha University |
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Principal Investigator |
HOJO TATSUYA 同志社大学, スポーツ健康科学部, 教授 (40298740)
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| Co-Investigator(Kenkyū-buntansha) |
IZAWA Tetsuya 同志社大学, スポーツ健康科学部, 教授 (70147495)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 関節軟骨 / 軟骨細胞 / 温熱刺激 / Heat Shock Protein 70 / 基質代謝 / アポトーシス / 温熱療法 / ヒートショックプロテイン / 軟骨基質代謝 / 物理療法 |
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| Outline of Final Research Achievements |
Cultured human articular chondrocytes were subjected to the 1st HS in a thermal bath (37, 39, 41, 43 °C for 20 min) after 48 h of differentiation. Then, 24 h after the 1st HS, half of them were exposed to fatal stress (2nd HS at 45 °C) for 15 min. Cell viability was measured 8 h after 2nd HS. Twenty-four hours after each HS, Hsp70, proteoglycan, and type II collagen mRNA expressions were quantified by rt-PCR.
After the 1st HS, Hsp70 expression gradually increased with increase in temperature of the HS. Proteoglycan and type II collagen gradually increased until HS at 41 °C. HSP70 expression level significantly increased 24 h after the 1st HS at 41 °C compared with that in untreated chondrocytes. Moreover, chondrocytes pretreated at 41 °C showed increased cartilage matrix gene expression and the most tolerance to the 2nd HS. Appropriate HS caused increase in HSP70 expression, leading to enhancement of chondrocytes’ tolerance to heat stress.
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