| Project/Area Number |
25462319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
Onodera Jun 北海道大学, 大学病院, 助教 (90374511)
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| Co-Investigator(Kenkyū-buntansha) |
近藤 英司 北海道大学, 医学研究科, 特任教授 (60374724)
安田 和則 北海道大学, －, 理事・副学長 (20166507)
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| Research Collaborator |
HOSAKA Midori 北海道大学, 医学研究科, 実験助手
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 運動器外傷学 / 腱損傷 / 腱マトリクス / マクロファージ |
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| Outline of Final Research Achievements |
The role played by macrophage migration inhibitory factor (MIF) in the process of wound healing is controversial. In this study, we hypothesized that the deficiency in MIF gene might delay tendon injury and bone-tendon healing in mice. Biomechanical testing showed that the structural properties were significantly lower in MIF gene-deficient mice (MIFKO) than in wild-type mice (WT). Histologically, healing tissues in MIFKO exhibited prolonged hypertrophy, poor vascularity, and prolonged increase in cell number compared with those in WT and low healing rate between grafted tendon and bone. Levels of matrix metalloproteinase (MMP)-13 mRNA in the healing tissue were significantly lower in MIFKO than in WT at 3 weeks after injury.
Taken together, it was suggested that MIFKO exhibited delayed healing of the tendon and the bone-grafted tendon junction.
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