An Exprerimental study of tumor associated gene espression through nuclear receptors in musculoskeretal malignant tumors.
| Project/Area Number |
25462321
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Akita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKADA KYOJI 秋田大学, 医学系研究科, 教授 (10185431)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 骨軟部腫瘍 / 脂溶性ビタミン / 核内受容体 / 肉腫 / 細胞分化 / 転写因子 |
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| Outline of Final Research Achievements |
In this study, we examined the effects of menaquinone (VK) and all-trans retinoic acid (ATRA) on the differentiation of the synovial sarcoma cell line HS-SY-II. Both VK and ATRA decreased mRNA expression of fatty acid binding protein 4 (FABP4) and inhibited the increase in mRNA expression of bone Gla protein (BGP). These effects of ATRA was slightly stronger than that of VK. However ATRA also increased mRNA expression of Runx2, PPARγ, Sox9 and COLA2, glycerolphosphate dehydrogenase (GPDH) mRNA expression was decreased on day 8 and 14. Our results suggested that both VK and ATRA induced the differentiation to osteoblasts and inhibited the differentiation to adipocytes in synovial sarcoma cell line HS-SY-II which is a stem cell malignancy.
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Report
(3 results)
Research Products
(1 results)
