Regulation of malignant bone and soft-tissue tumor metastasis and invasion via cell morphology, glucose metabolism and microenvironment.

Research Project

Project/Area Number	25462322
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Orthopaedic surgery
Research Institution	Gunma University
Principal Investigator	Yanagawa Takashi 群馬大学, 医学部附属病院, 講師 (40400725)
Research Collaborator	FUNASAKA Tatsuyoshi 金沢大学, フロンティアサイエンス機構 Raz Avraham Karmanos Cancer Institute
Project Period (FY)	2013-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords	細胞形態 / 細胞運動 / 転移 / 浸潤 / 肉腫 / AMF / 三次元培養 / 糖代謝 / 間葉性遊走 / アメーバ様遊走
Outline of Final Research Achievements	Human fibrosarcoma cell line HT-1080 shows both mesenchymal and amoeboid migrations in 3D collagen gel cell culture system. We revealed that autocrine motility factor (AMF) changed the cells showing mesenchymal shape into amoeboid type via RhoA activation. Glucose metabolism did not affect the morphological change, although AMF is also important for glucose metabolism not for cell migration.

Report

(4 results)