Detection of circulating tumor cells targeting tumor specific fusion gene in musculoskeletal sarcoma

• Project/Area Number: 25462325
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Niigata University
• Principal Investigator: Hotta Tetsuo 新潟大学, 医歯学総合病院, 准教授 (00272815)
• Co-Investigator(Kenkyū-buntansha): KAWASHIMA Hiroyuki 新潟大学, 医歯学系, 講師 (30361900)
• Research Collaborator: SASAKI Taro ; YAMAGISHI Tetsuro
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 血中循環微量腫瘍細胞 / 骨軟部肉腫 / 融合遺伝子 / 遺伝子増幅 / 定量的RT-PCR

Outline of Final Research Achievements

We developed a new technique to detect the specific gene expression in musculoskeletal sarcoma cells. Highly purified RNA was extracted from the 2 mL blood sample contaminated with 5 sarcoma cells. The condition of gene amplification was optimized and the target gene could be sensitively detected. Furthermore, the target gene was also detected in the blood sample from a patient who carried lung metastasis of musculoskeletal sarcoma.

Research Products

• [Journal Article] Effect of temozolomide on the viability of musculoskeletal sarcoma cells. (2015)
  • Author(s): Kusabe Y, Kawashima H, Ogose A, Sasaki T, Ariizumi T, Hotta T, Endo N.
  • Journal Title: Oncology letters Volume: 10 Pages: 2511-2518
  • Peer Reviewed / Open Access
• [Journal Article] Telomelysin shows potent antitumor activity through apoptotic and non-apoptotic cell death in soft tissue sarcoma cells. (2013)
  • Author(s): Li GD, Kawashima H, Ogose A, Ariizumi T, Hotta T, Kuwano R, Urata Y, Fujiwara T, Endo N
  • Journal Title: Cancer Science Volume: 104 Issue: 9 Pages: 1178-1188
  • DOI: 10.1111/cas.12208
  • Peer Reviewed
• [Presentation] 軟部腫瘍における治療標的としてのRANKL関連分子の発現解析 (2015)
  • Author(s): 山岸 哲郎, 生越 章, 川島 寛之, 佐々木 太郎, 堀田 哲夫, 遠藤 直人, 梅津 哉, 畠野 宏史, 有泉 高志
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山国際会議場（富山県・富山市）
  • Year and Date: 2015-10-22
• [Presentation] 脂肪腫と高分化型脂肪肉腫に対する針生検での微量検体RNAを用いたreal-time PCRによる遺伝子解析 (2013)
  • Author(s): 佐々木 太郎, 生越 章, 川島 寛之, 有泉 高志, 堀田 哲夫, 田中 恵子, 遠藤 直人
  • Organizer: 第46回日本整形外科学会 骨・軟部腫瘍学術集会
  • Place of Presentation: 東京