Analysis of Osteochondroma Formation Using Prx1Cre ERT; Ext1flox/flox mice

Research Project

Project/Area Number	25462326
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Orthopaedic surgery
Research Institution	Gifu University
Principal Investigator	Ito Yoshiki 岐阜大学, 医学(系)研究科(研究院), 非常勤講師 (10313884)
Co-Investigator(Kenkyū-buntansha)	Kazu Matsumoto 岐阜大学, 医学系研究科, 准教授 (40422711)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	多発性外骨腫症
Outline of Final Research Achievements	Multiple hereditary exostosis (MHE) is autosomal dominant disorder characterized by multiple osteochondroma formation and deformities of extremities due to osteochondroma formation. To determine the function of heparin sulfate in the perichondrium, we have generated Prx1-CreER; Ext1flox/flox mice (Cre mice) by breeding the Prx1-CreER transgenic mice with the Ext1flox/flox mice. EXT1 expression was deleted in the perichondrium due to tamoxifen injection after birth, and Cre mice exhibited the osteochondroma formation in the extremities.