Target gene screening of TGF-b signaling and functional analysis for differentiation-inducing therapy of chondrosarcoma
| Project/Area Number |
25462343
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Maeda Shingo 鹿児島大学, 医歯(薬)学総合研究科, 特任准教授 (60353463)
Komiya Setsuro 鹿児島大学, 医歯学域医学系, 教授 (30178371)
Ishidou Yasuhiro 鹿児島大学, 医歯(薬)学総合研究科, 特任准教授 (10300740)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 軟骨肉腫 / 内軟骨腫 / TGF-b / 分化度 / 鑑別分子マーカー / TGF-β / PEG10 |
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| Outline of Final Research Achievements |
It is one of the major dilemma for orthopaedic oncologists that, because histology of low grade chondrosarcoma and its benign counterpart enchondroma resembles, there are difficulties in distinguishing these two tumors. Our aim of this study was to discover molecular markers specific for each type of tumor. Moreover, we intended to understand underlying mechanism in lowered differentiation status of chondrosarcoma, to develop differentiation-initiating therapy. We focused on TGF-b signaling, because it is a crucial pathway in chondrocyte differentiation. By immunohistochemistry for phosphorylated SMAD2/3, the TGF-b signaling intracellular mediators, we found malignant grading-dependent increase of TGF-b signaling. We searched for genes which expression was altered by TGF-b in chondrosarcoma to find PEG10 to be down-regulated by the pathway. The combined expression analysis of p-SMAD2/3 and PEG10 may help distinguishing and diagnosing these chondrogenic tumors.
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Report
(3 results)
Research Products
(9 results)
