Development of new multiplex PCR assay for preiprosthetic joint infection
| Project/Area Number |
25462346
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INABA Yutaka 横浜市立大学, 附属病院, 准教授 (40336574)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 人工関節周囲感染 / 診断 / PCR / リアルタイムPCR / 迅速診断 |
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| Outline of Final Research Achievements |
Molecular diagnostic techniques can contribute to the diagnosis of periprosthetic joint infection (PJI). We have developed a new multiplex real-time polymerase chain reaction (PCR) assay. The most prominant feature of our PCR assay is specific detection of methicillin-resistant staphylococcus (MRS) and distinguish between Gram-positive and -negative bacteria.
This novel multiplex real-time PCR system has several advantages compared to previously reported methods and has potential uses in the diagnosis of PJIs, particularly those caused by viable but non-culturable organisms.
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Report
(3 results)
Research Products
(7 results)
