| Project/Area Number |
25462351
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nara Medical University |
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Principal Investigator |
Murata Keiichi 奈良県立医科大学, 医学部, 研究員 (10382318)
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| Co-Investigator(Kenkyū-buntansha) |
赤羽 学 奈良県立医科大学, 医学部, 准教授 (40326327)
田中 康仁 奈良県立医科大学, 医学部, 教授 (30316070)
清水 隆昌 奈良県立医科大学, 医学部, 助教 (70464667)
面川 庄平 奈良県立医科大学, 医学部, 教授 (70597103)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 再生医療 / 間葉系幹細胞 / βTCP / 血管 / 骨新生 / 血管誘導 / 間葉性幹細胞 / 血管新生 |
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| Outline of Final Research Achievements |
We developed a novel vascularized tissue-engineered bone (VTEB). Using rat models, a vascular bundle of femoral artery and vein was passed through the groove of the β-tricalcium phosphate ceramics (βTCP). Osteogenic matrix cell sheets (OMCS) wrapped around vascular bundles within βTCP. Four weeks after the implantation of the vascular pedicle, the VTEB was elevated as a single vascular peddled VTEB, implanted to the same site wrapping with a silastic sheet to prevent blood supply from surrounding tissue. Additional 4 weeks after this manipulation, the VTEB was harvested to evaluate angiogenic and osteogenic potentials. As a results, the VTEB combined with OMCS maintained a high angiogenic and osteogenic potential even after elevation as a pedicled VTEB. From the results of this study, the vascularized βTCP combined with OMCS is expected to be a powerful tool of bone reconstruction for the treatment of intractable nonunions with massive bone defects.
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