Investigation of molecular mechanisms of chondrogenesis by platelet rich plasma
| Project/Area Number |
25462358
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Research Collaborator |
MISHIMA Yuko
TAKAYANAGI Syunsaku
KAWABATA Michiru
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 多血小板血漿 / クリオプレシピテート / 軟骨分化 / 転写因子 / PDGFβ / EMX / SOX / 輸血療法 / 選択的スプライシング / ＳＯＸトリオ / 血小板由来増殖因子 |
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| Outline of Final Research Achievements |
Platelet rich plasma (PRP) and cryoprecipitate induced chondrogenesis and cell growth into un-differentiated chondrocytic cell line ATDC5 and human mesenchymal stem cells (MSC). This data has shown a possibility that cryoprecipitate will be applicable for cartilage regeneration medicine as well as PRP. SOX6 and SOX9 played important roles on in vitro chondrogenesis induced by PRP or cryoprecipitate. PDGFβ was also identified as a causal molecule of cartilage differentiation and cell growth induced by PRP. We found that EMX1 and EMX2 were induced by SOX6 and SOX9 and could induce cartilage differentiation into ATDC5 and MSC.
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Report
(3 results)
Research Products
(6 results)
