Investigation of molecular mechanisms of chondrogenesis by platelet rich plasma

• Project/Area Number: 25462358
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokyo
• Principal Investigator: Ikeda Toshiyuki 東京大学, 医学部附属病院, 助教 (80322759)
• Research Collaborator: MISHIMA Yuko ; TAKAYANAGI Syunsaku ; KAWABATA Michiru
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 多血小板血漿 / クリオプレシピテート / 軟骨分化 / 転写因子 / PDGFβ / EMX / SOX / 輸血療法 / 選択的スプライシング / ＳＯＸトリオ / 血小板由来増殖因子

Outline of Final Research Achievements

Platelet rich plasma (PRP) and cryoprecipitate induced chondrogenesis and cell growth into un-differentiated chondrocytic cell line ATDC5 and human mesenchymal stem cells (MSC). This data has shown a possibility that cryoprecipitate will be applicable for cartilage regeneration medicine as well as PRP. SOX6 and SOX9 played important roles on in vitro chondrogenesis induced by PRP or cryoprecipitate. PDGFβ was also identified as a causal molecule of cartilage differentiation and cell growth induced by PRP. We found that EMX1 and EMX2 were induced by SOX6 and SOX9 and could induce cartilage differentiation into ATDC5 and MSC.

Research Products

• [Journal Article] Hedgehog-Gli activators direct osteo-chondrogenic function of bone morp hogenetic protein toward osteogenesis in the perichondrium. (2013)
  • Author(s): Hojo H, et al.
  • Journal Title: J Biol Chem Volume: 288 Issue: 14 Pages: 9924-9932
  • DOI: 10.1074/jbc.m112.409342
  • Peer Reviewed
• [Journal Article] A novel disease-modifying osteoarthritis drug candidate targeting Runx1 (2013)
  • Author(s): Yano, F. et al.
  • Journal Title: Ann Rheum Dis. Volume: 72 Issue: 5 Pages: 748
  • DOI: 10.1136/annrheumdis-2012-201745
  • Peer Reviewed
• [Presentation] 脳神経外科手術における自己血輸血の安全性・有効性 その１ (2016)
  • Author(s): 高柳俊作、池田敏之 他
  • Organizer: 日本自己血輸血学会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-03-11
• [Presentation] 脳神経外科手術における自己血輸血の安全性・有効性 その２ (2016)
  • Author(s): 川端みちる、池田敏之 他
  • Organizer: 日本自己血輸血学会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-03-11
• [Presentation] Identification and characterization of molecular basis of Del mutations in the Japanese population (2015)
  • Author(s): Mishima Y, Sato T, Ikeda T et al.
  • Organizer: AABB
  • Place of Presentation: Anaheim
  • Year and Date: 2015-10-24
  • Int'l Joint Research
• [Remarks] 東京大学医学部附属病院 輸血部
  • URL: http://square.umin.ac.jp/traf-tky/