Differential gene expression of mesenchymal stem cells derived from bone-marrow and synovial-tissues

• Project/Area Number: 25462362
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: EZURA Yoichi 東京医科歯科大学, 難治疾患研究所, 准教授 (50333456)
• Co-Investigator(Renkei-kenkyūsha): SEKIYA Ichiro 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (10345291)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 間葉系幹細胞 / 組織間質細胞 / 間葉系前駆細胞 / 軟骨細胞分化能 / 滑膜由来細胞 / 骨髄由来細胞 / 半月板由来細胞 / 遺伝子発現

Outline of Final Research Achievements

Appropriate "quality check (QC)" of mesenchymal stem cells (MSC) for regenerative therapy requires practical and quantitative marker set. Here, to establish such marker set, we investigated the profiles of gene expression in human MSCs derived from bone-marrow and synovial-tissues. By clarifying that mouse stromal progenitor cells derived from bone-marrow and synovial-tisues also showed similar differential pattern of the gene expression to that observed in human MSCs, we explored the marker set that would predict the in vitro chondrogenic potentials of the MSCs and stromal progenitor cells. Also, the potential for the unwanted ectopic calcification was evaluated by in vitro culture assays and gene expression.

Research Products

• [Journal Article] Interleukin-1β Suppresses the Transporter Genes Ank and Ent1 Expression in Stromal Progenitor Cells Retaining Mineralization. (2016)
  • Author(s): Ezura Y, Lin X, Hatta A, Izu Y, Noda M.
  • Journal Title: Calcif Tissue Int. Volume: -
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Interleukin-1β suppresses expression of osteoblastic genes as well as the regulators of ecto-nucleotides and pyrophosphate that negatively regulate bio-mineralization in mouse bone marrow stromal cells (2015)
  • Author(s): Ezura Y, Hatta A, Lin Xin, Yayoi Izu, Hayata T, Noda M.
  • Organizer: ASBMR annual meeting
  • Place of Presentation: Seatle, USA
  • Year and Date: 2015-10-08
  • Int'l Joint Research
• [Presentation] Genes significantly highly expressed in synovium derived stromal cells than in bone marrow derived cells are conserved both in mouse and human, and may contribute to higher potential for chondrogenic differentiation. (2013)
  • Author(s): Ezura Y, Hayata T, Notomi T, Sekiya I, Noda M.
  • Organizer: American Society for Bone Mineral and Research annual meeting,
  • Place of Presentation: ボルチモア（米国）
• [Presentation] Identification of differentially expressed genes in mesenchymal stem cells derived from synovium, meniscus and ligament. (2013)
  • Author(s): Ezura Y, Hayata T, Notomi T, Noda M.
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
  • Place of Presentation: 神戸