| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We implanted Gellan-GS soaked in TNC into full-thickness osteochondral defects of the patellar groove of rabbits. Histologically as well as macroscopically, we found satisfactory repair of cartilage. Using mice, full-thickness osteochondral defects were created and the defects were filled with TNC. Histologic study showed satisfactory repair of cartilage. In posttraumatic osteoarthritis model with transection of anterior cruciate ligament and medial collateral ligament, we performed intra-articular injection of TNC. TNC administration markedly protected the articular cartilage from proteoglycan depletion for 6 weeks. These studies indicated that TNC could promote cartilage repair and protect cartilage degeneration in vivo. Although TNC upregulated the expression of ADAMTS4, TNC downregulated the expression of ADAMTS5.
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