| Project/Area Number |
25462371
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
UCHIO Yuji 島根大学, 医学部, 教授 (20223547)
KUWATA Suguru 島根大学, 医学部, 助教 (80509000)
KONO Michihaya 島根大学, 医学部, 助教 (30547740)
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| Research Collaborator |
OSAGO Harumi 島根大学, 医学部, 技術専門職員
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | グリコサミノグリカン / 質量分析 / 軟骨 / 靭帯 / 骨・軟骨代謝学 / GAG / gagomic |
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| Outline of Final Research Achievements |
We developed a method for quantitative analysis of four classes of glycosaminoglycans (GAGs) using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). We demonstrated the quantification of a total of 29 saccharides (23 repeating disaccharides, 3 terminal mono- and disaccharides and 3 linkage hexasaccharides). The method with efficient extractions of GAGs from human cartilage, ligament and tendon can be used for clinical GAGomic analysis.
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