| Project/Area Number |
25462379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Saitama Medical University |
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Principal Investigator |
Araki Yasuto 埼玉医科大学, 医学部, 講師 (10580839)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 関節リウマチ / 変形性関節症 / MMP / エピジェネティクス / ヒストン修飾 / IL-6 / MMP分子 / 関節炎 / エピゲノム |
|---|
| Outline of Final Research Achievements |
To clarify dysfunctions of epigenetic regulation in the pathogenesis of rheumatiod arthritis (RA), gene expression of matrix metalloproteinases (MMPs), which are matrix-degrading enzymes, and histone modifications in the promoters were examined in RA synovial fibroblasts (SFs) and osteoarthritis (OA) SFs. Gene expression of MMP-1, 3, 9, and 13 was significantly higher in RASFs than in OASFs. Histone methylation profiles in the MMP promoters were consistent with the MMP gene expression. In addition, MMP-1, 3, and 13 genes were responsive to IL-6 stimulation in RASFs. Taken together, it is suggested that histone methylation plays an important role in the activation of RASFs.
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