Molecular analysis of role of miRNA in bone metabolism

Research Project

Project/Area Number	25462381
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Orthopaedic surgery
Research Institution	Tokyo Medical and Dental University
Principal Investigator	Fukuda Toru 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (20301492)
Co-Investigator(Renkei-kenkyūsha)	TAKEDA Shu 東京医科歯科大学, 大学院, 教授 (30376727)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	マイクロRNA / RNAヘリカーゼ / 骨代謝
Outline of Final Research Achievements	MicroRNAs (miRNAs) are small non-coding RNAs that regulate the expression of the target protein by inhibiting the translation or accelerating the degradation of the transcript through binding to the 3’-UTR of those target genes. MiRNAs are important regulators in various developmental, physiological and pathological conditions. Previous reports suggested that miRNAs are also intimately involved in the osteoblast and osteoclast differentiation.In this study, we identified miR-382 as an up-regulated miRNA during osteoblast differentiation. We discovered that miR-382 stimulates osteoblast differentiation by targeting Prps2 and Ddx3x.