The experiment to examine the effect of anesthetic agents on injured neurons

• Project/Area Number: 25462407
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Yokohama City University
• Principal Investigator: ANDOH Tomio 横浜市立大学, 医学(系)研究科(研究院), 客員教授 (00193110)
• Co-Investigator(Kenkyū-buntansha): KAMIYA Yoshinori 新潟大学, 医師学総合病院, 教授 (90381491)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 神経障害性疼痛 / 神経因性疼痛 / KCC2 / 神経損傷 / GABAA受容体 / 鎮静薬 / NKCC1 / アポトーシス

Outline of Final Research Achievements

The neuropathic pain model rats exhibit pain behaviour in 2 to 3 weeks after surgery. The phenomenon that down regulation of KCC2 and relative up regulation of NKCC1 has been proposed as promising mechanism underlying pain behaviour. We examined two models, SNL and CCI. While SNL rats did not exhibit the down regulation of KCC2, CCI showed the down regulation of pKCC2 in spinal dorsal horn, which is consistent with pain behaviour examined with weight distribution test. However the administration of CLP290, KCC2 activator, did not rescue pain behaviour.