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Study on activation of the descending pain inhibitory systems in a neuropathic pain rat model

Research Project

Project/Area Number 25462448
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Anesthesiology
Research InstitutionOsaka City University

Principal Investigator

Nishikawa Kiyonobu  大阪市立大学, 大学院医学研究科, 教授 (20145791)

Research Collaborator FANAI Yusuke  大阪市立大学, 大学院医学研究科, 大学院生 (60722486)
YAMASAKI Hiroyuki  大阪市立大学, 大学院医学研究科, 大学院生 (70759000)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords下行性抑制系 / in vivo patch clamp / 脊髄 / トラマドール / DSP-4 / 交感神経 / デクスメデトミジン
Outline of Final Research Achievements

We examined the role of the descending pain inhibitory system in a rat spinal nerve ligation model (Chung model) by analyzing behavioral experiments together with synaptic responses using spinal cord in vivo patch clamp method. Tramadol decreased spontaneous excitatory synaptic currents (sIPSC) and increased spontaneous inihibitory synaptic currents (sIPSC), but preparation of semi-resection of cervical cord for resection of spinal descending inihibitory pathway revealed that M1, the major metabolite of tramadol, acts at supraspinal site.
Destruction of the noradrenaline neurons at the locus coeruleus did not bring about a reverse phenomenon of allodynia at the left and the right side, therefore we were unable to confirm a mechanism that excitation of the inhibitory neurons inversely potentiated pain.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (19 results)

All 2016 2015 2014 2013

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Levobupivacaine - dextran mixture for transversus abdominis plane block and rectus sheath block in patients undergoing laparoscopic colectomy: a randomised controlled trial.2016

    • Author(s)
      Hamada T, Tsuchiya M, Mizutani K, Takahashi R, Muguruma K, Maeda K, Ueda W, Nishikawa K.
    • Journal Title

      Anaesthesia

      Volume: 71 Issue: 4 Pages: 411-416

    • DOI

      10.1111/anae.13408

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effects of tramadol on substantia gelatinosa neurons in the rat spinal cord: an in vivo patch-clamp analysis.2015

    • Author(s)
      Hiroyuki Yamasaki, Yusuke Funai, Tomoharu Funao, Takashi Mori, Kiyonobu Nishikawa
    • Journal Title

      PLOS ONE

      Volume: 印刷中 Issue: 5 Pages: e0125147-e0125147

    • DOI

      10.1371/journal.pone.0125147

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Systemic dexmedetomidine augments inhibitory synaptic transmission in the superficial dorsal horn through activation of descending noradrenergic control : an in vivo patch-clamp analysis of analgesic mechamisms2014

    • Author(s)
      Funai Y, Pickering AE, Uta D, Nishikawa K, Mori T, Asada A, Imoto K and Furue H
    • Journal Title

      Pain

      Volume: 155(3) Issue: 3 Pages: 617-628

    • DOI

      10.1016/j.pain.2013.12.018

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The effect of lipid emulsion on intracellular bupivacaine as a mechanism of lipid resuscitation: An electrophysiological study using voltage-gated proton channels2013

    • Author(s)
      Kotaro Hori, Tadashi Matsuura, Takashi Mori, Miyuki Kuno, Makoto Sawada, Kiyonobu Nishikawa
    • Journal Title

      Anesthesia and Analgesia

      Volume: 117 Pages: 1293-1301

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Systemic α2 agonist administration facilitates inhibitory synaptic transmission in the rat spinal dorsal horn mediated through α1 adrenoceptors2013

    • Author(s)
      Yusuke Funai, Kiyonobu Nishikawa, Takashi Mori, Akira Asada, Keiji Imoto, Hidemasa Furue
    • Journal Title

      Pain Research

      Volume: 28 Pages: 145-153

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Relationship between noradrenaline release in the locus coeruleus and antiallodynic efficacy of analgesics in rats with painful diabetic neuropathy2013

    • Author(s)
      Koichi Suehiro, Tomoharu Funao, Yohei Fujimoto, Tokuhiro Yamada, Takashi Mori, Kiyonobu Nishikawa.
    • Journal Title

      Life Sciences

      Volume: 92(23) Issue: 23 Pages: 1138-44

    • DOI

      10.1016/j.lfs.2013.04.015

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 肩関節手術後鎮痛 ー麻酔科からの視点ー2013

    • Author(s)
      西川精宣
    • Journal Title

      整形・災害外科

      Volume: 56 Pages: 1561-1565

    • Related Report
      2013 Research-status Report
  • [Presentation] 神経障害性疼痛は交感神経β2受容体を介して酸化ストレスに心筋保護的に作用する2016

    • Author(s)
      河合茂明, 山﨑広之, 萩原千恵, 山田徳洪, 西川精宣
    • Organizer
      日本麻酔科学会第63回学術集会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市博多区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] アミトリプチリン中毒に対する脂肪乳剤の有効性―アルカリ化療法との比較―2016

    • Author(s)
      辻川翔吾, 松浦正, 堀耕太郎, 森隆, 久野みゆき, 西川精宣
    • Organizer
      日本麻酔科学会第63回学術集会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市博多区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Neuropathic Pain Diminishes Cellular Damages at Ischemia-reperfusion Injury in Cardiac Myocytes2015

    • Author(s)
      Shigeaki Kawai, Hiroyuki Yamasaki, Tokuhiro Yamada, Kiyonobu Nishikawa
    • Organizer
      2015 Annual Meeting, American Society of Anesthesiologists
    • Place of Presentation
      San Diego Convention Center, San Diego, USA
    • Year and Date
      2015-10-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 全身麻酔手術における胸部硬膜外麻酔の術直後心室再分極への影響:無作為化比較試験による検討2015

    • Author(s)
      辻川翔吾, 堀耕太郎, 舟井優介, 松浦正, 森隆, 西川精宣
    • Organizer
      日本麻酔科学会第62回学術集会
    • Place of Presentation
      神戸ポートピアホテル、神戸
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] ブピバカインのリピッドレスキューにおける電気生理学的効果の検討2015

    • Author(s)
      堀耕太郎, 松浦正, 辻川翔吾, 森隆, 久野みゆき, 西川精宣
    • Organizer
      日本麻酔科学会第62回学術集会
    • Place of Presentation
      神戸ポートピアホテル、神戸
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Lipid rescueにおける”lipid sink"の効果発現時間についてー電位依存性プロトンチャンネルによる検討2013

    • Author(s)
      堀耕太郎、松浦 正、羽阪めぐみ、森 隆、久野みゆき、西川精宣
    • Organizer
      日本麻酔科学会第60回学術集会
    • Place of Presentation
      ロイトン札幌、 札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] ジアゼパムはリドカイン・トラマドール誘発性痙攣をともに抑制する2013

    • Author(s)
      藤本陽平、舟尾友晴、末廣浩一、高橋陵太、西川精宣
    • Organizer
      日本麻酔科学会第60回学術集会
    • Place of Presentation
      ロイトン札幌、札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] 脊髄後角in vivoパッチクランプ法によるトラマドールの鎮痛機序の検討2013

    • Author(s)
      山崎広之、森 隆、舟井優介、濱田 拓、山田徳洪、西川精宣
    • Organizer
      日本麻酔科学会第60回学術集会
    • Place of Presentation
      ロイトン札幌、札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] Antinociceptive actions of tramadol revealed by in vivo patch-clamp recordings in the spinal dorsal horn2013

    • Author(s)
      Hiroyuki Yamasaki, Takashi Mori, Yusuke Funai, Taku Hamada, Tokuhiro Yamada, Kiyonobu Nishikawa
    • Organizer
      Euroanesthesia 2013
    • Place of Presentation
      Centre de Convencions Internacional de Barcelona, Barcelona, Spain
    • Related Report
      2013 Research-status Report
  • [Presentation] The recovery speed of lipid emulsion therapy largely depends on "lipid sink": An electrophysiological study using voltage-gated proton channels2013

    • Author(s)
      Kotaro Hori, Tadashi Matsuura, Megumi Hasaka, Takashi Mori, Miyuki Kuno, Kiyonobu Nishikawa
    • Organizer
      Euroanesthesia 2013
    • Place of Presentation
      Centre de Convencions Internacional de Barcelona, Barcelona, Spain
    • Related Report
      2013 Research-status Report
  • [Presentation] Brain serotonin content regulates manifestation of tramadol-induced seizure in rats2013

    • Author(s)
      Yohei Fujimoto, Tomoharu Funao, Koichi Suehiro, Ryota Takahasi, Katsuaki Tanaka, Kiyonobu Nishikawa
    • Organizer
      2013 Annual Meeting, American Society of Anesthesiologists
    • Place of Presentation
      Moscone Convention Center, San Francisco, California, USA
    • Related Report
      2013 Research-status Report
  • [Book] 麻酔科医のための周術期の疼痛管理;新戦略に基づく麻酔・周術期医学 6章 周術期疼痛の治療法 6-3 術後痛に有効なその他の薬物2014

    • Author(s)
      西川精宣 専門編集:川真田樹人、監修:森田 潔
    • Total Pages
      320
    • Publisher
      中山書店
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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