Elucidation of mechanism of neuropathic pain using acromelic acid A-induced allodynia model
Project/Area Number |
25462456
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Anesthesiology
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Research Institution | Osaka Medical College |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
森本 賢治 大阪医科大学, 医学部, 助教 (20388250)
藤原 俊介 大阪医科大学, 医学部, 講師 (60535813)
中平 淳子 大阪医科大学, 医学部, 助教 (30465603)
駒澤 伸泰 大阪医科大学, 医学部, 助教 (20529226)
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Co-Investigator(Renkei-kenkyūsha) |
FURUTA Kyoji 岐阜大学, 医学(系)研究科(研究院), 准教授 (40173538)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Keywords | 神経障害性疼痛 / アロディニア / グルタミン酸受容体 / アクロメリン酸 / アストロサイト / ミクログリア / がん性疼痛 / グリア / 神経障害性痛 / 脊髄 |
Outline of Final Research Achievements |
We previously showed that intrathecal administration of acromelic acid A (ACRO-A) provoked tactile allodynia in mice. In order to clarify their involvement in ACRO-A-induced allodynia, we investigated the effects of various agents at early and late-phase allodynia. All of agents (Ca2+ channel α2δ ligands, NMDA and AMPA receptor antagonists, nNOS, and Ca2+/calmodulin kinase II inhibitors) blocked allodynia in the early-phase group; however, they did not block allodynia in the late-phase group. In order to block glial activation, astrocytic inhibitor LAA or microglia linhibitor minocycline was administrated. Microglial activation was observed 1week after ACRO-A injection and was significantly inhibited with minocycline treatment. Moreover, only LAA was found to inhibit late-phase allodynia. We demonstrate that NMDA receptor activation is involved only in ACRO-A-induced allodynia in the early phase, and that spinal astrocytic activation contributes to allodynia in the late phase.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Involvement of NIPSNAP1, a neuropeptide nocistatin-interacting protein, in inflammatory pain.2016
Author(s)
Okamoto K, Ohashi M, Ohno K, Takeuchi A, Matsuoka E, Fujisato K, Minami T, Ito S, Okuda-Ashitaka E.
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Journal Title
Mol. Pain
Volume: 12
Pages: 1-11
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Cerebrospinal fluid of postherpetic neuralgia patients induced interleukin-6 release in human glial cell-line T98G.2013
Author(s)
Tay, A.S., Liu, E.H., Lee, T.L., Miyazaki, S., Nishimura, W., Minami, T., Chan, Y.H., Low, C-M., Tachibana, S.
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Journal Title
Neurochem. Intl.
Volume: 63
Issue: 5
Pages: 517-521
DOI
Related Report
Peer Reviewed
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[Journal Article] The action site of the synthetic kainoid (2S,3R,4R)-3-carboxymethyl-4-(4-methylp henylthio) pyrrolidine-2-carboxylic acid (PSPA-4), an analogue of Japanese mushroom poison acromelic acid, for allodynia (tactile pain)2012
Author(s)
Miyazaki S, Minami T, Mizuma H, Kanazawa, M, Doi H, Matsumura S, Lu J, Onoe H, Furuta K, Suzuki M and Ito S
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Journal Title
Eur J Pharmacol
Volume: (in press)
Issue: 1-3
Pages: 120-127
DOI
Related Report
Peer Reviewed
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[Presentation] アクロメリン酸類縁体のPETプローブ化
Author(s)
小岩大智, 金澤将勝, 宮崎信一郞, 水間 広, 土居久志, 尾上浩隆, 鈴木正昭, 南 敏明, 伊藤誠二, 古田享史
Organizer
第44回中部化学関係学協会支部連合秋季大会
Place of Presentation
浜松
Related Report
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