| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
This study aimed to recover function degeneracy of immune cells under hypothermia by use of scientific thermal stimulation. The experiment was conducted by isolating neutrophils from a healthy adult subject. Chemotactic activity of the neutrophils was confirmed to lower as the temperature declined. However, scientific thermal stimulation through activation of neutrophil TRPV1 by an agonist did not improve the chemotactic activity. In addition, chemotactic activity of the neutrophils exposed to an anesthetic was confirmed to show nearly no change. Elastase-releasing capacity of the neutrophils also lowered with decline of the temperature similarly as chemotactic activity, but showed no improvement after TRPV1 stimulation. The above indicated that TRPV1 stimulation cannot be a thermal stimulus that improves functions of neutrophils. However, involvement of other temperature-sensitive TRP channels is left for future investigation.
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