| Project/Area Number |
25462461
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Koji 産業医科大学, 医学部, 非常勤医師 (50248572)
SATA Takeyoshi 産業医科大学, 大学病院, 病院長 (60128030)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 外科的侵襲 / 免疫 / 抗菌ぺプチド / 外科侵襲 / 感染症 / 免疫学 / 抗菌ペプチド / 自然免疫 |
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| Outline of Final Research Achievements |
Immunosuppressive neutrophils (PMN-II) appeared in association with surgical injury play a role on the increased susceptibility of patient to various infections. In the present study, a role of PMN-II on the production of beta-defensins (BD), the important molecules on the host antimicrobial innate immunities, by human keratinocytes was studied. Normal human epidermal keratinocytes were transwell-cultured with neutrophils, isolated from surgical patients or normal volunteers. The production of BD by NHEK was suppressed by PMN from surgical patients. The culture fluids of NHEK cultured with surgical patient PMN showed decreased antimicrobial activities, as compared with controls. PMN isolated from peripheral blood of surgical patients were confirmed as PMN-II, because these cells produced CCL2, but not IL-12 and CCL3. These results indicate that PMN-II appeared in association with surgical injury contribute to the decreased production of BD in surgically injured patients.
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