Impact of microRNAs on high-fat diet induced prostate cancer progression

• Project/Area Number: 25462466
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Akita University
• Principal Investigator: Narita Shintaro 秋田大学, 医学部, 講師 (40396552)
• Co-Investigator(Kenkyū-buntansha): INOUE Takamitsu 秋田大学, 医学部, 講師 (60375243) ; HUANG Mingguo 秋田大学, 医学部, 助教 (60448503)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 前立腺癌 / microRNA / 高脂肪食 / MET / miR-130a

Outline of Final Research Achievements

We assessed the roles of altered miRNAs in HFD-induced PCa progression. We found 59 up- and down-regulated miRNAs in xenografts under the HFD condition using microRNA Array analysis. In those miRNAs, we focused on miR-130a because of the expression levels with significantly lower in three PCa cells. In ex vivo, PCa cells cultured in medium containing HFD-fed mouse serum had significantly higher cell proliferation with lower expression levels of miR-130a. The PCa cells transfected with miR130a down-regulated MET, AR and DICER1, and MET expression was higher in HFD-induced PCa models. In human tissues, the mean miR-130a expression level of cancer epithelia was significantly lower than that of normal epithelia, and cytoplasmic MET in PCa tissues overexpressed in patients with higher BMI. In conclusion, a substantial number of miRNAs altered in HFD-induced PCa growth. In particular, miR-130a/MET pathway was associated with HFD-induced PCa progression.

Research Products

• [Journal Article] Diet-induced alteration of fatty acid synthase in prostate cancer progression (2016)
  • Author(s): Huang M, Koizumi A, Narita S, Inoue T, Tsuchiya N, Nakanishi H, Numakura K, Tsuruta H, Saito M, Satoh S, Nanjo H, Sasaki T, Habuchi T
  • Journal Title: Oncogenesis Volume: ５ Issue: 2 Pages: e195-e195
  • DOI: 10.1038/oncsis.2015.42
  • Peer Reviewed / Open Access
• [Presentation] miR-130aはMET高発現を伴い高脂肪食摂取下の前立腺癌増殖に関与する (2016)
  • Author(s): 奈良健平
  • Organizer: 第25会分子・細胞研究会
  • Place of Presentation: 大阪
  • Year and Date: 2016-02-27
• [Presentation] シンポジウム前立腺癌進展の分子メカニズムと去勢抵抗性前立腺癌に対する治療戦略：前立腺癌発症・進展における高脂肪食および脂質代謝の役割 (2015)
  • Author(s): 成田伸太郎
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] Candidate microRNAs involved in high-fat diet induced tumor progression in prostate cancer (2013)
  • Author(s): Taketoshi Nara
  • Organizer: Annual meeting of American Urological Association
  • Place of Presentation: San Diego