Development of a novel diagnostic method for bladder cancer
| Project/Area Number |
25462471
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kanagawa Cancer Center Research Institute (2015)
The University of Tokyo (2013) |
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Principal Investigator |
Koshikawa Naohiko 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), その他部局等, 部長 (70334282)
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| Co-Investigator(Renkei-kenkyūsha) |
SHUIN Taro 高知大学, 副学長 (70128601)
KAMADA Masayuki 高知大学, 医学部泌尿器科学教室, 非常勤講師 (90304683)
KARASHIMA Takashi 高知大学, 医学部泌尿器科学教室, 講師 (60304672)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | バイオマーカー / 診断 / 治療奏効 / 膀胱がん / 癌 / 細胞外マトリックス / トランスレーショナルリサーチ |
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| Outline of Final Research Achievements |
In this study we analyzed serum monomeric lamininγ2 (Ln-γ2) as a biomarker for urological cancers. First, we established a quantitative sandwich ELISA using a specific antibody to monomeric Ln-γ2, and was able to measure 50 pg/mL of monomeric Ln-γ2 in serum specimens. After screening using serum specimens obtained from patient with urological cancers, the high levels of monomeric Ln-γ2 was observed in serum of bladder and renal cancers.
Furthermore, to assess whether a specific antibody to monomeric Ln-γ2 as a DDS tool for bladder cancer therapy, we established an intravesical model with bladder cancer cells expressing monomeric Ln-γ2 in nude mice. Now the specific antibody is immunohistochemically assessing in the mouse model with HT-1197 cell expressing high level of endogenous monomeric Ln-γ2.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Proteolysis of EphA2 Converts It from a Tumor Suppressor to an Oncoprotein. Cancer Res2015
Author(s)
Koshikawa N, Hoshino D, Taniguchi H, Minegishi T, Tomari T, Nam SO, Aoki M, Sueta T, Nakagawa T, Miyamoto S, Nabeshima K, Weaver AM, Seiki M
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Journal Title
Cancer Res
Volume: 75(16)
Issue: 16
Pages: 3327-39
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Tropomodulin 1 expression driven by NF-kB enhances breast cancer growth.2015
Author(s)
Ito-Kureha, T., Koshikawa, N., Yamamoto, M., Semba, K., Yamaguchi, N., Yamamoto, T., Seiki, M. and Inoue, J.
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Journal Title
Cancer Res
Volume: 75
Issue: 1
Pages: 62-72
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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