| Project/Area Number |
25462471
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kanagawa Cancer Center Research Institute (2015)
The University of Tokyo (2013) |
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Principal Investigator |
Koshikawa Naohiko 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), その他部局等, 部長 (70334282)
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| Co-Investigator(Renkei-kenkyūsha) |
SHUIN Taro 高知大学, 副学長 (70128601)
KAMADA Masayuki 高知大学, 医学部泌尿器科学教室, 非常勤講師 (90304683)
KARASHIMA Takashi 高知大学, 医学部泌尿器科学教室, 講師 (60304672)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | バイオマーカー / 診断 / 治療奏効 / 膀胱がん / 癌 / 細胞外マトリックス / トランスレーショナルリサーチ |
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| Outline of Final Research Achievements |
In this study we analyzed serum monomeric lamininγ2 (Ln-γ2) as a biomarker for urological cancers. First, we established a quantitative sandwich ELISA using a specific antibody to monomeric Ln-γ2, and was able to measure 50 pg/mL of monomeric Ln-γ2 in serum specimens. After screening using serum specimens obtained from patient with urological cancers, the high levels of monomeric Ln-γ2 was observed in serum of bladder and renal cancers.
Furthermore, to assess whether a specific antibody to monomeric Ln-γ2 as a DDS tool for bladder cancer therapy, we established an intravesical model with bladder cancer cells expressing monomeric Ln-γ2 in nude mice. Now the specific antibody is immunohistochemically assessing in the mouse model with HT-1197 cell expressing high level of endogenous monomeric Ln-γ2.
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