| Project/Area Number |
25462475
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Mie University |
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Principal Investigator |
Ishii Kenichiro 三重大学, 医学(系)研究科(研究院), 助教 (90397513)
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| Co-Investigator(Kenkyū-buntansha) |
Shiraishi Taizo 三重大学, 大学院医学系研究科, 教授 (30162762)
Hirokawa Yoshifumi 三重大学, 大学院医学系研究科, 講師 (30322738)
Sugimura Yoshiki 三重大学, 大学院医学系研究科, 教授 (90179151)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 前立腺癌 / 間質リモデリング / 細胞競合 / 線維芽細胞 / 平滑筋細胞 / 癌関連線維芽細胞 / 共培養実験 / 去勢抵抗性前立腺癌 / in vitro共培養系 / 増殖因子 / サイトカイン |
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| Outline of Final Research Achievements |
Carcinoma-associated fibroblasts (CAFs) are activated fibroblasts that support the proliferation and invasion of adjacent cancer cells. In the tumor microenvironment, CAFs communicate not only with cancer cells but also surrounding stromal cells. In this study, we performed in vitro co-culture experiments using commercially available normal human prostate stromal cells PrSC and primary cultures of human prostate fibroblasts (pcPrFs) derived from prostate cancer patients. In PrSC, protein productions of TGFb1, IL-6, and VEGF were significantly increased by co-culturing with pcPrFs. Similarly, those of IL-6 and VEGF in pcPrFs were significantly increased by co-culturing with PrSC. In PrSC, mRNA expressions of growth factor were significantly increased by co-culturing with pcPrFs. Those of growth factor in pcPrFs were significantly increased by co-culturing with PrSC. Here our results demonstrated that interactions between PrSC and pcPrFs showed quite heterogeneous.
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