Badder instillation treatment of periostin in a mouse orthotopic model of bladder cancer

• Project/Area Number: 25462476
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Kim Tetsusyo 滋賀医科大学, 医学部, 非常勤講師 (10204968)
• Co-Investigator(Kenkyū-buntansha): INOUE Hirokazu 滋賀医科大学, 医学部, 准教授 (30176440)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Periostin / 膀胱癌 / 膀胱内注入療法 / 浸潤能 / periostin

Outline of Final Research Achievements

Periostin is an extracellular matrix protein involved in the regulation of intercellular adhesion. We investigated the in vivo tumor suppressor function of periostin in a mouse orthotopic model of bladder cancer. Vector-control UMUC-3 bladder tumors had histological evidence of muscle invasion. However, the periostin-expressing UMUC-3 bladder tumors did not reveal muscle invasion with thick edematous lesions in the submucosa. Phosphorylation of PDK-1, Akt, and S6 ribosomal protein was decreased in periostin-expressing UMUC-3 cells compared with vector-control cells. Treatment with 100 ng/mL recombinant human periostin protein also suppressed cell invasiveness and phosphorylation of PDK-1, Akt, and S6 in UMUC-3 cells. Periostin suppresses in vivo and in vitro invasiveness of bladder cancers via the PDK1/Akt/mTOR signaling pathway. Periostin have utility as a potent chemotherapeutic agent by suppressing bladder cancer invasiveness.

Research Products

• [Journal Article] Akt-dependent activation of Erk by cyclin D1b contributes to cell invasiveness and tumorigenicity. (2016)
  • Author(s): Kim CJ, Tambe Y, Mukaisho K, Sugihara H, Kawauchi A, Inoue H
  • Journal Title: Oncol Lett Volume: 11
  • Peer Reviewed
• [Journal Article] Periostin suppresses in vivo invasiveness via PDK1/Akt/mTOR signaling pathway in a mouse orthotopic model of bladder cancer. (2016)
  • Author(s): Kim CJ, Tambe Y, Mukaisho K, Sugihara H, Kageyama S, Kawauchi A, Inoue H
  • Journal Title: Oncol Lett Volume: 11
  • Peer Reviewed
• [Journal Article] Female-specific rectal carcinogenesis in cyclin D1b transgenic mice. (2014)
  • Author(s): Tambe, Y., Kim, C. J., Munkaisho, K., Sugihara, H., Isono, T., Sonoda, H., Shimizu, T., Kondoh, G., Inoue, H.
  • Journal Title: Carcinogenesis Volume: 35 Issue: 1 Pages: 227-236
  • DOI: 10.1093/carcin/bgt293
  • Peer Reviewed
• [Journal Article] Diagnostic criteria for stomal obstruction of tubeless cutaneous ureterostomy using 99mTc-mercaptoacetyltriglycine (MAG3) diuretic renography (2013)
  • Author(s): Chul Jang Kim, Shigehisa Kubota, Ryosuke Murai
  • Journal Title: Korean J Urol Volume: 54 Issue: 5 Pages: 322-326
  • DOI: 10.4111/kju.2013.54.5.322
  • Peer Reviewed
• [Presentation] Effect of inhibitors for LDH and PDK4 on malignant phenotypes of mouse and human cancer cell lines (2015)
  • Author(s): Tambe Y, Kim CJ, Inoue H
  • Organizer: 74th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-10
• [Presentation] Periostin suppresses invasiveness and tumorigenicity via Akt/mTOR pathway in a mouse orthotopic model of bladder cancer. (2015)
  • Author(s): Kim CJ, Tambe Y, Mukaisho K, Sugihara H, Kawauchi A, Inoue H
  • Organizer: 74th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-09
• [Presentation] マウス膀胱癌同所性モデルを用いたperiostinの抗腫瘍効果の検討 (2015)
  • Author(s): 金 哲將、旦部幸博、河内明宏、井上寛一
  • Organizer: 第103回日本泌尿器科学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2015-04-18
• [Presentation] Molecular mechanism of female-specific rectal carcinogenesis in cyclin D1b transgenic mice
  • Author(s): Chul Jang Kim, Yukihiro Tambe, Ken-ichi Mukaisho, Hiroyuki Sugihara, Hirokazu Inoue
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] Cyclin D1bトランスジェニックマウスにおける雌特異的直腸腫瘍発生の分子機構
  • Author(s): 旦部幸博, 金 哲將, 向所賢一, 杉原洋行, 井上寛一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸市