| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Periostin is an extracellular matrix protein involved in the regulation of intercellular adhesion. We investigated the in vivo tumor suppressor function of periostin in a mouse orthotopic model of bladder cancer. Vector-control UMUC-3 bladder tumors had histological evidence of muscle invasion. However, the periostin-expressing UMUC-3 bladder tumors did not reveal muscle invasion with thick edematous lesions in the submucosa. Phosphorylation of PDK-1, Akt, and S6 ribosomal protein was decreased in periostin-expressing UMUC-3 cells compared with vector-control cells. Treatment with 100 ng/mL recombinant human periostin protein also suppressed cell invasiveness and phosphorylation of PDK-1, Akt, and S6 in UMUC-3 cells. Periostin suppresses in vivo and in vitro invasiveness of bladder cancers via the PDK1/Akt/mTOR signaling pathway. Periostin have utility as a potent chemotherapeutic agent by suppressing bladder cancer invasiveness.
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