Development of a novel technique for the detection and isolation of disseminated cancer cells, and establishment of its application foundation

Research Project

Project/Area Number	25462478
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Urology
Research Institution	Okayama University
Principal Investigator	UEKI HIDEO 岡山大学, 医学部, 技術専門職員 (90537218)
Co-Investigator(Kenkyū-buntansha)	NASU Yasutomo 岡山大学, 大学院医歯薬学総合研究科, 教授 (20237572)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	腫瘍 / 細胞検出
Outline of Final Research Achievements	The purpose of this study is to conduct foundational research for development of a novel technique for the detection and isolation of disseminated cancer cells with our original gene expression systems. The reporter gene, GFP expression under the system was investigated in various cancer and normal cells and it was confirmed that the system can increase the cancer cell-specific gene expression. It was also confirmed that the GFP encoding plasmid system allowed in vitro selective visualization of floating human cancer cells in mixed cell culture containing 10000-fold more normal blood cells.

Report

(3 results)