| Project/Area Number |
25462489
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MIMATA HIROMITSU 大分大学, 医学部, 教授 (60219714)
NOMURA TAKEO 大分大学, 医学部, 講師 (40347034)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 前立腺癌 / 浸潤 / invasive front / マウスモデル / microRNA / 発現解析 / invasive frint / in vivo モデル / micro RNA / in vivoモデル / 局所浸潤 |
|---|
| Outline of Final Research Achievements |
The aim of this study is to elucidate the molecular mechanism of prostate cancer cell invasion using micro-RNA (miRNA) expression analysis. The LNCaP, PC-3 or DU145 prostate cancer cell was inoculated in nude mouse. The PC-3 reproducibly showed local invasion. Immunohistochemical analysis showed cancer cells in invasive front significantly decreased phosphorylated E-cadherin and beta-catenin, and increased phosphorylated Akt, and MMP-7 and 9. The miRNA expression pattern at the invasive front was different from that of non-invasive part of cancer, several microRNA were identified to be a potential regulator of cancer invasion. Further investigation should be done to answer this principal question.
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