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Inhibitory effects of carbonic anhydrase 2 inhibitor on the invasion of urinary bladder cancer

Research Project

Project/Area Number 25462499
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionOsaka City University

Principal Investigator

GI Min  大阪市立大学, 大学院医学研究科, 准教授 (70336783)

Co-Investigator(Kenkyū-buntansha) TAMADA Satoshi  大阪市立大学, 大学院医学研究科, 講師 (20382179)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords膀胱癌 / 炭酸脱水酵素2阻害薬 / 炭酸脱水酵素2阻害剤 / carbonic anhydrase 2 / urinary bladder cancer / Invasion
Outline of Final Research Achievements

We have demonstrated that that carbonic anhydrase 2 (CA2) is an invasion-associated factor in urothelial carcinoma (UC). The purpose to the present study is to examine the inhibitory effects of CA inhibitor Acetazolamide (Ace) on the N-butyl-N-(hydroxybutyl)nitrosameine (BBN)-induced mouse urinary bladder carcinogenesis. We found that concurrent treatment with Ace and Cisplatin (Cis) significantly inhibited the development of BBN-induced UC. Moreover, muscle invasive UC were significantly decreased by the Ace alone and concurrent treatment with Ace and Cis. These findings indicate that CA could serve as a potential therapeutic molecular target for bladder cancers and suggest that CA inhibitors are useful for prevention of UC invasion.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (32 results)

All 2016 2015 2014 2013

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (20 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] The role of deltaNp63pos CD44vpos cells in the development of NTCU-induced peripheral type of mouse lung squamous cell carcinomas..2016

    • Author(s)
      Yamano S, Gi M, Tago Y, Doi K, Okada S, Hirayama S, Tachibana H, Ishii N, Fujioka M, Tatsumi K,Wanibuchi H.
    • Journal Title

      Cancer Sci

      Volume: 107 Issue: 2 Pages: 123-132

    • DOI

      10.1111/cas.12855

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CD44v9 is a potential biomarker of tumor initiating cells predicting survival outcome in HCV-positive patients with resected HCC.2016

    • Author(s)
      Kakehashi A, Ishii N, Sugihara E, Gi M, Saya H, Wanibuchi H.
    • Journal Title

      Cancer Sci

      Volume: 印刷中 Issue: 5 Pages: 609-618

    • DOI

      10.1111/cas.12908

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of non-genotoxic hepatocarcinogens and prediction of their mechanism of action in rats using gene marker sets2016

    • Author(s)
      Kanki M, Gi M, Fujioka M, Wanibuchi H.
    • Journal Title

      The Journal of Toxicological Sciences

      Volume: 41 Issue: 2 Pages: 281-292

    • DOI

      10.2131/jts.41.281

    • NAID

      130005132661

    • ISSN
      0388-1350, 1880-3989
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Integrative analyses of miRNA and proteomics identify potential biological pathways associated with onset of pulmonary fibrosis in the bleomycin rat model.2015

    • Author(s)
      Fukunaga S, Kakehashi A, Sumida K, Kushida M, Asano H, Gi M, Wanibuchi H
    • Journal Title

      Toxicol Appl Pharmacol

      Volume: 286 Issue: 3 Pages: 188-198

    • DOI

      10.1016/j.taap.2015.04.014

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Induction of cell proliferation in the rat liver by the short-term administration of ethyl tertiary-butyl ether.2015

    • Author(s)
      Kakehashi A, Hagiwara A, Imai N, Wei M, Fukushima S, Wanibuchi H.
    • Journal Title

      J Toxicol Pathol

      Volume: 28 Issue: 1 Pages: 27-32

    • DOI

      10.1293/tox.2014-0056

    • NAID

      130004842605

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Ethanol-extracted propolis enhances BBN-initiated urinary bladder carcinogenesis via non-mutagenic mechanisms in rats.2015

    • Author(s)
      Xie XL, Gi M, Fujioka M, Doi K, Yamano S, Tachibana H, Fang H, Kakehashi A, Wanibuchi H.
    • Journal Title

      Food Chem Toxicol

      Volume: 83 Pages: 193-200

    • DOI

      10.1016/j.fct.2015.06.007

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modifying effects of 1,2-dichloropropane on N-nitrosobis(2-oxopropyl)amine-induced cholangiocarcinogenesis in male Syrian hamsters2015

    • Author(s)
      Gi M, Fujioka M, Yamano S, Shimomura E, Kanki M, Kawachi S, Tachibana H, Tatsumi K, Fang H, Ishii N, Kakehashi A, Wanibuchi H.
    • Journal Title

      The Journal of Toxicological Sciences

      Volume: 40 Issue: 5 Pages: 647-656

    • DOI

      10.2131/jts.40.647

    • NAID

      130005098383

    • ISSN
      0388-1350, 1880-3989
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Diphenylarsinic acid, a chemical warfare-related neurotoxicant, promotes liver carcinogenesis via activation of aryl hydrocarbon receptor signaling and consequent induction of oxidative DNA damage in rats2013

    • Author(s)
      Wei M (9人), et al
    • Journal Title

      Toxicol Appl Pharmacol

      Volume: 273(1) Issue: 1 Pages: 1-9

    • DOI

      10.1016/j.taap.2013.08.022

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mode of action of ethyl tertiary-butyl ether hepatotumorigenicity in the rat : evidence for a role of oxidative stress via activation of CAR, PXR and PPAR signaling pathways2013

    • Author(s)
      Kakehashi A,Wei M(9人中7番目), et al
    • Journal Title

      Toxicol Appl Pharmacol

      Volume: 273(2) Issue: 2 Pages: 390-400

    • DOI

      10.1016/j.taap.2013.09.016

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel medium-term carcinogenesis model for lung squamous cell carcinoma induced by N-nitroso-tris-chloroethylurea in mice2013

    • Author(s)
      Tago Y, Yamano S, Wei M, Kakehashi A., Kitano M., Fujioka M., Ishii N., Wanibuchi H.
    • Journal Title

      Cancer Sci

      Volume: 104 Issue: 12 Pages: 1560-1566

    • DOI

      10.1111/cas.12289

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] L-Leucine and L-isoleucine enhance growth of BBN-induced urothelial tumors in the rat bladder by modulating expression of amino acid transporters and tumorigenesis-associated genes2013

    • Author(s)
      Xie XL, Kakehashi A, Wei M, et al.
    • Journal Title

      Food Chem Toxicol

      Volume: 59 Pages: 137-144

    • DOI

      10.1016/j.fct.2013.05.044

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Mode of Action of Ethyl Tertiary-butyl Ether in the Rat Liver: Induction of Oxidative Stress, Cell Proliferation and Cell Cycle Arrest via Activation of CAR, PXR and PPARs.2016

    • Author(s)
      Kakehashi A, Gi M, Wanibuchi H, Hagiwara A, Imai M, Nagano K, Nishimaki F, Fukushima S
    • Organizer
      The 55th Annual Meeting of the Society of Toxicology
    • Place of Presentation
      New Orleans(USA)
    • Year and Date
      2016-03-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 非遺伝毒性ラット肝発がん物質ダンマル樹脂の発がん機序の検討2016

    • Author(s)
      藤岡正喜、魏 民、山野荘太郎、河内聡子、房 赫、武下正憲、鰐渕英機
    • Organizer
      第32回日本毒性病理学会総会及び学術集会
    • Place of Presentation
      サンポール高松(香川県高松市)
    • Year and Date
      2016-01-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mcl-1蛋白を標的とする低分子抗がん物質の毒性と有効性の評価2016

    • Author(s)
      土井賢一郎、鰐渕英機、魏 民、梯アンナ、石井真美、山野荘太郎
    • Organizer
      第32回日本毒性病理学会総会及び学術集会
    • Place of Presentation
      サンポール高松(香川県高松市)
    • Year and Date
      2016-01-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] メタボリックシンドロームモデルマウス(TSODマウス)における肝腫瘍の発生メカニズムの検討2016

    • Author(s)
      石井真美、梯アンナ、辰己久美子、藤岡正喜、土井賢一郎、魏 民、鰐渕英機
    • Organizer
      第32回日本毒性病理学会総会及び学術集会
    • Place of Presentation
      サンポール高松(香川県高松市)
    • Year and Date
      2016-01-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] CD44ノックアウトマウスを用いた二段階発がん性試験における肝臓腫瘍の発生の検討2016

    • Author(s)
      梯アンナ、石井真美、辰己久美子、魏 民、佐谷秀行、鰐渕英機
    • Organizer
      第32回日本毒性病理学会総会及び学術集会
    • Place of Presentation
      サンポール高松(香川県高松市)
    • Year and Date
      2016-01-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] CYP2E1ノックアウトマウスを用いた1,2-ジクロロプロパンの肝細胞毒性機序の検討2016

    • Author(s)
      河内聡子、藤岡正喜、辰己久美子、魏 民、山野荘太郎、房 赫、武下正憲、鰐渕英機
    • Organizer
      第32回日本毒性病理学会総会及び学術集会
    • Place of Presentation
      サンポール高松(香川県高松市)
    • Year and Date
      2016-01-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] ダンマル樹脂の雄性F344ラットにおける肝発がん性2016

    • Author(s)
      房 赫、魏 民、藤岡正喜、辰己久美子、河内聡子、土井賢一郎、石井真美、梯アンナ、山野荘太郎、鰐渕英機
    • Organizer
      第32回日本毒性病理学会総会及び学術集会
    • Place of Presentation
      サンポール高松(香川県高松市)
    • Year and Date
      2016-01-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] ダンマル樹脂のF344ラットを用いたがん原性試験:ダンマル樹脂はラット肝発がん物質である2015

    • Author(s)
      魏 民、山野荘太郎、藤岡正喜、梯アンナ、立花大和、土井賢一郎、鰐渕英機
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] ダンマル樹脂のラット肝発がんメカニズムの検討2015

    • Author(s)
      藤岡正喜、魏 民、山野荘太郎、房 赫、河内聡子、武下正憲、鰐渕英機
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヌードマウス皮下腫瘍モデルにおけるCNPY2およびCACHD1ノックダウウンにより肝発がんの抑制2015

    • Author(s)
      梯アンナ、石井真美、楠元久美子、藤岡正喜、魏 民、鰐渕英機
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] ラットモデルを用いた食道腺癌発癌におけるマイクロバイオメータの意義2015

    • Author(s)
      沢田明也、藤原靖弘、魏 民、鰐渕英機、荒川哲男
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] ラット肝発がんにおけるValerianの抑制作用2015

    • Author(s)
      石井真美、梯アンナ、魏 民、鰐渕英機
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 実験的アプローチを用いた食品中ヒ素のリスク評価2015

    • Author(s)
      魏 民、藤岡正喜、立花大和、鰐渕英機
    • Organizer
      第59回日本薬学会関東支部大会
    • Place of Presentation
      日本大学薬学部(千葉県船橋市)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 遺伝子発現データによる短期肝発がん物質検出系の開発2015

    • Author(s)
      神吉将之、藤岡正喜、山野荘太郎、梯アンナ、魏 民、鰐渕英機
    • Organizer
      第30回発癌病理研究会
    • Place of Presentation
      リゾートホテル オリビアン小豆島(香川県小豆郡土庄町)
    • Year and Date
      2015-08-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膀胱癌予防のためのリスク因子の同定と発がん機序の解明2015

    • Author(s)
      鰐渕英機、魏 民、梯アンナ、立花大和
    • Organizer
      第24回日本腎泌尿器疾患予防医学研究会
    • Place of Presentation
      ホテルニューオータニ大阪(大阪府大阪市)
    • Year and Date
      2015-07-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ラットにおける2-エトキシ-2-メチルプロパンの肝臓発腫瘍性機序の解明2015

    • Author(s)
      梯アンナ、萩原昭裕、今井則夫、魏 民、福島昭治、鰐渕英機
    • Organizer
      第42回日本毒性学会学術年会
    • Place of Presentation
      石川県立音楽堂(石川県金沢市)
    • Year and Date
      2015-06-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒト肝細胞癌における新規分子マーカーとしてCNPY2およびCACHD1の同定2015

    • Author(s)
      梯アンナ、桑江優子、石井真美、魏 民、藤岡正喜、鰐渕英機
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-04-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] プロテオーム解析によるヒトNASH肝細胞癌の分子メカニズムの検討2015

    • Author(s)
      石井真美、梯アンナ、楠元久美子、藤岡正喜、魏 民、桑江優子、大澤政彦、鰐渕英機
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-04-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Diphenylarsinic acid, a chemical warfare-related neurotoxicant, promotes liver carcinogenesis via activation of aryl hydrocarbon receptor signaling and consequent induction of oxidative DNA damage in rats.2014

    • Author(s)
      Wei M, Yamano S, Fujioka M, Kakehashi A, Wanibuchi H
    • Organizer
      平成25年度個体レベルでのがん研究支援活動ワークショップ
    • Place of Presentation
      大津 琵琶湖ホテル
    • Related Report
      2013 Research-status Report
  • [Presentation] シリアンハムスターにおける1,2‐dichrolopropanの強制経口投与による肝毒性とその発現機序の検討2013

    • Author(s)
      魏 民、山野荘太郎、藤岡正喜、梯アンナ、下村衣里、神吉将之、鰐渕英機
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜 パシフィコ横浜
    • Related Report
      2013 Research-status Report
  • [Book] 細胞工学 免疫組織染色の定量法2014

    • Author(s)
      藤岡正喜、山野荘太郎、魏 民、鰐渕英機
    • Total Pages
      7
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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