| Project/Area Number |
25462505
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Hokkaido University |
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Principal Investigator |
Kitta Takeya 北海道大学, 大学病院, 講師 (40374441)
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| Co-Investigator(Kenkyū-buntansha) |
MITSUI TAKAHIKO 山梨大学, 総合研究部, 講師 (90421966)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 排尿反射 / 脳 / 神経伝達物質 / セロトニン / 神経解剖学 / 排尿障害 / ラット |
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| Outline of Final Research Achievements |
Microdyalysis in the prefrontalcortex (PFC) and cystometrography (CMG) were performed under consciousness. Experiment 1: Extracellular neurotransmitters were collected by microdyalysis. At the same time, CMG were performed to measure intercontraction interval (ICI) and maximum voiding pressure (MVP). Experiment 2: SSRI was administered into the PFC. Experiment 3: Following SSRI administration, 5-HT1A agonist, which has the effect of decreasing the level of serotonin (5-HT) in the PFC, was administered into the PFC. Ex. 1: Extracellular level of 5-HT in the PFC significantly increased during micturition reflex, whereas levels of glutamate or dopamine were not significantly changed. Ex. 2: The 5-HT level increased. It also significantly increased ICI, whereas no significant change was found in MVP. Ex. 3: The 5-HT decreased, thereby ICI significantly decreased. The results suggest that the PFC has a suppressive effect on neural control of the micturition reflex via serotonin.
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