Change of bladder microcirculation in bladder outlet obstruction and effect of alpha-1 adrenoceptor blockers

• Project/Area Number: 25462510
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Nagoya University
• Principal Investigator: Gotoh Momokazu 名古屋大学, 医学(系)研究科(研究院), 教授 (10186900)
• Co-Investigator(Kenkyū-buntansha): Funahashi Yasuhito 名古屋大学, 大学院医学系研究科, 助教 (70534824)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 膀胱虚血再灌流障害 / 下部尿路閉塞 / 交感神経アルファ1受容体 / 交感神経アルファ1遮断薬 / アルファ受容体サブタイプ / 膀胱微小循環 / 膀胱虚血再還流障害 / アルファ1受容体サブタイプ / 交感神経α1遮断薬

Outline of Final Research Achievements

Bladder microcirculation in the condition of ischemia-reperfusion was analyzed using a pencil lens CCD microscopy system, in bladder ischemia-reperfusion model rats and bladder outlet obstruction model rats. It was demonstrated that the etiology of bladder storage dysfunction would be blood flow impairment due to microcirculation damage caused by bladder over distension. These microcirculation damage was suppressed through alpha-1A adrenoceptor subtype by pretreatment with alpha-1 blockers. The present results suggested that development of new medicine to improvement of bladder microcirculation targeting on alpha-1A adrenoceptor subtype would be of use for treatment of storage dysfunction due to bladder outlet obstruction.

Research Products

• [Journal Article] Effect of naftopidil on bladder microcirculation in a rat model of bladder outlet obstruction. (2015)
  • Author(s): Majima T, Yamamoto T, Funahashi Y, Takai S, Matsukawa Y, Yoshida M, Gotoh M
  • Journal Title: LUTS Volume: epub on ahead Issue: 2 Pages: 111-116
  • DOI: 10.1111/luts.12119
  • Peer Reviewed / Int'l Joint Research
• [Presentation] The imaging of leakage of red blood cells from submucosal capillary after bladder overdistension effect of alpha 1 blocker for hematuria after acute urinary retention. (2015)
  • Author(s): Muzuno H, Funahashi Y, Matsukawa Y, Yamamoto T, Gotoh M, Muramatsu T, Soeda Y, Okamoto N, Kimura K, Aota Y
  • Organizer: 45th Annual Meeting of International Continence Society
  • Place of Presentation: Montreal/Canada
  • Year and Date: 2015-10-07
  • Int'l Joint Research
• [Presentation] The effect of silodosin on the prostatic microcirculation in a prostatitis rat model induced by urine-reflux. (2015)
  • Author(s): Funahashi Y, Yoshida M, Yamamoto T, Matsukawa Y, Majima T, Gotoh M
  • Organizer: 45th Annual Meeting of International Continence Society
  • Place of Presentation: Montreal/Canada
  • Year and Date: 2015-10-07
  • Int'l Joint Research
• [Presentation] 急性尿閉解除後の血尿：膀胱過伸展・過伸展解除後（膀胱虚血再灌流障害）の膀胱微小循環 (2015)
  • Author(s): 水野秀紀、山本徳則、村松知昭、副田雄也、岡本典子、木村恭祐、岡村菊夫、青田泰博、後藤百万
  • Organizer: 第22回日本排尿機能学会
  • Place of Presentation: 京王プラザホテル札幌（北海道札幌市中央区）
  • Year and Date: 2015-09-10
• [Presentation] Effect of naftopidil on bladder microcirculation in a bladder outlet obstruction rat model: Evaluation with a pencil lens charged-coupled device microscopy system
  • Author(s): Tsuyoshi Majima, Yasuhito Funahashi，Shun Takaiｉ，Yoshihisa Matsukawa，Tokunori Yamamoto，Momokazu Gotoh
  • Organizer: Annual Meeting of American Urological Association
  • Place of Presentation: サンディエゴ、米国