| Project/Area Number |
25462519
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nagoya City University |
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Principal Investigator |
Sakakura Takeshi 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (00275132)
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| Co-Investigator(Kenkyū-buntansha) |
KOHRI Kenjiro 名古屋市立大学, 学長 (30122047)
TOZAWA Keiichi 名古屋市立大学, 大学院医学研究科, 准教授 (40264733)
YASUI Takahiro 名古屋市立大学, 大学院医学研究科, 教授 (40326153)
OKADA Atsushi 名古屋市立大学, 大学院医学研究科, 講師 (70444966)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 腎結石 / メタボリックシンドローム / 接着 / ob/ob / 細胞接着分子 |
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| Outline of Final Research Achievements |
There was no stone formation in WT mice, but significant renal crystal formation around the cortico-medullary junction was observed in ob/ob mice on day 6. Gene ontology analysis showed significant inter-group differences, with the highest ratio in the molecular function category of adhesion (68.6%, p = 1.25 × 10-9). Furthermore, 4 adhesion-related genes, Spp1, Fn1, Icam1, and Col3a1, showed significant inter-group differences in expression in the microarray analysis. The expression levels of these 4 genes on day 6 were significantly higher in ob/ob mice than in WT mice (p < 0.05) by quantitative PCR. The protein expression levels of these 4 factors were also significantly higher in ob/ob than in WT mice by immunohistochemical staining. The present study showed that MetS-associated renal crystal formation increased the expression of atherosclerosis-related factors, indicating that cell adhesion plays an important role in renal crystal formation as well as in arteriosclerosis.
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