Effects of atherosclerosis-related factors on renal crystal formation in a mouse model of metabolic syndrome
| Project/Area Number |
25462519
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nagoya City University |
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Principal Investigator |
Sakakura Takeshi 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (00275132)
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| Co-Investigator(Kenkyū-buntansha) |
KOHRI Kenjiro 名古屋市立大学, 学長 (30122047)
TOZAWA Keiichi 名古屋市立大学, 大学院医学研究科, 准教授 (40264733)
YASUI Takahiro 名古屋市立大学, 大学院医学研究科, 教授 (40326153)
OKADA Atsushi 名古屋市立大学, 大学院医学研究科, 講師 (70444966)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 腎結石 / メタボリックシンドローム / 接着 / ob/ob / 細胞接着分子 |
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| Outline of Final Research Achievements |
There was no stone formation in WT mice, but significant renal crystal formation around the cortico-medullary junction was observed in ob/ob mice on day 6. Gene ontology analysis showed significant inter-group differences, with the highest ratio in the molecular function category of adhesion (68.6%, p = 1.25 × 10-9). Furthermore, 4 adhesion-related genes, Spp1, Fn1, Icam1, and Col3a1, showed significant inter-group differences in expression in the microarray analysis. The expression levels of these 4 genes on day 6 were significantly higher in ob/ob mice than in WT mice (p < 0.05) by quantitative PCR. The protein expression levels of these 4 factors were also significantly higher in ob/ob than in WT mice by immunohistochemical staining. The present study showed that MetS-associated renal crystal formation increased the expression of atherosclerosis-related factors, indicating that cell adhesion plays an important role in renal crystal formation as well as in arteriosclerosis.
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Report
(3 results)
Research Products
(31 results)
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[Presentation] Association between nephrolithiasis incidence and nutrition, based on data from Japanese national surveys.2013
Author(s)
Yasui Takahiro, Okada Atsushi, Zuo Li, Hirose Yasuhiko, Taguchi Kazumi, Kobayashi Takahiro, Hirose Masahito, Ando Ryosuke, Sasaki Shoichi, Kohri Kenjiro
Organizer
American Urological Association Annual Meeting
Place of Presentation
San Diego Convention Center( San Diego, USA)
Related Report
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