| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We evaluate the relationship between urolithiasis and High Mobility Group Box1 (HMGB1) and Receptor of Advanced of Glycation Endoproduct (RAGE).
Renal epithelial HK-2 and MDCK cells were obtained and maintained. Calcium oxalate monohydrate (COM) crystals were suspended in serum-free medium at a final concentration of 500μg/ml. The total protein was extracted by phenol/ammonium acetate in methanol method, and total RNA was extracted by the acid guanidium thiocyanate-phenol-chloroform method. Western blot study revealed the expression of HMGB1. Expression of HMGB1 and monocyte chemoattractant protein-1 (MCP-1), which is induced by interaction between renal epithelial cells and COM crystals was detected by reverse transcription-polymerase chain reaction (RT-PCR). MCP-1 genes were also up-regulated with exposure of COM crystal.
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