| Project/Area Number |
25462535
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Osaka University |
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Principal Investigator |
ICHIMARU Naotsugu 大阪大学, 医学(系)研究科(研究院), 寄附講座准教授 (70346211)
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| Co-Investigator(Kenkyū-buntansha) |
LI Xiao-Kang 独立行政法人国立成育医療研究センター, 移植免疫研究室, 室長 (60321890)
TAKAHARA Shiro 大阪大学, 大学院医学系研究科, 寄附講座教授 (70179547)
KAIMORI Jun-ya 大阪大学, 大学院医学系研究科, 寄附講座准教授 (70527697)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 免疫寛容 / CD40 siRNA / siCD40/SPG複合体 / siCD40 / SPG |
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| Outline of Final Research Achievements |
The CD40/CD154 co-stimulatory pathway is crucial in alloimmune response. We developed a small interfering RNA (siRNA) delivery system. This was captured and incorporated into dendritic cells (DCs) through its receptor, Dectin-1, specifically silencing CD40 genes (siCD40) to exert immunoregulatory activity.
As a result, siCD40/SPG-treated CBA mice permanently accepted B10 fully mismatched cardiac allografts. In addition, naive CBA recipients given an adoptive transfer of splenocytes from the primary recipients with siCD40/SPG accepted a heart graft from donor-type B10, but not third-party Balb/c mice.
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