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Elucidation of the epigenetic regulatory network during cell differentiation of spermatogonial stem cells

Research Project

Project/Area Number 25462541
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionNagoya City University

Principal Investigator

Azemoto Masataka  名古屋市立大学, 医学(系)研究科(研究院), 研究員 (70264736)

Co-Investigator(Kenkyū-buntansha) KAMISAWA HIDEYUKI  名古屋市立大学, 大学院医学研究科, 研究員 (00551277)
NISHIO HIDENORI  名古屋市立大学, 大学院医学研究科, 助教 (10621063)
KOHRI KENJIRO  名古屋市立大学, 学長 (30122047)
HAYASHI YUTARO  名古屋市立大学, 大学院医学研究科, 准教授 (40238134)
KOJIMA YOSHIYUKI  福島県立医科大学, 医学部, 教授 (60305539)
水野 健太郎  名古屋市立大学, 医学(系)研究科(研究院), 講師 (70448710)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords精子幹細胞 / エピゲノム / 精巣腫瘍 / 細胞分化
Outline of Final Research Achievements

Recently, epigenetic regulation has benn regarded as an important system of gene regulation. There are DNA methylation or histone modification as typical regulatory system, and it seems that epigenome is involved in the determination of cell fate. From the results of our previous work, we considered that the dysfunction of spermatogonial stem cells is one of the reasons why spermatogenesis is disrupted in cryptorchidism. In the present research, we aimed the specific epigenetic regulatory network during cell differentiation of spermatogonial stem cells. We assessed the spermatogonial stem cells in the testes of each developmental stages using experimental animals. As a result, we detected that Kdm5a, demethylase for histone H3K4, and miR-135a (microRNA) are involved in differentiation of spermatogonial stem cells.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (18 results)

All 2015 2014 2013

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Acknowledgement Compliant: 1 results,  Open Access: 3 results) Presentation (9 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Novel splice site mutation in MAMLD1 in a patient with hypospadias.2015

    • Author(s)
      10.1038/ejhg.2015.13
    • Journal Title

      Sex Dev

      Volume: 3 Issue: 3 Pages: 1-1

    • DOI

      10.1159/000380842

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Clear cell sarcoma of the kidney distinguished from synovial sarcoma of the kidney by genetic analysis2015

    • Author(s)
      Hirose M, Mizuno K, Kamisawa H, Nishio H, Moritoki Y, Kohri K, Hayashi Y.
    • Journal Title

      BMC Research Notes

      Volume: 8 Issue: 1 Pages: 129-133

    • DOI

      10.1186/s13104-015-1100-5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Proinflammatory and Metabolic Changes Facilitate Renal Crystal Deposition in an Obese Mouse Model of Metabolic Syndrome.2015

    • Author(s)
      Taguchi K, Okada A, Hamamoto S, Iwatsuki S, Naiki T, Ando R, Mizuno K, Tozawa K, Kohri K, Yasui T.
    • Journal Title

      J Urol.

      Volume: 194 Issue: 6 Pages: 1787-96

    • DOI

      10.1016/j.juro.2015.07.083

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] First case report of staghorn calculi successfully removed by mini-endoscopic combined intrarenal surgery in a 2-year-old boy.2015

    • Author(s)
      Taguchi Kazumi, Hamamoto Shuzo, Okada Atsushi, Mizuno Kentaro, Tozawa Keiichi, Hayashi Yutaro, Kohri Kenjiro, Yasui Takahiro
    • Journal Title

      International Journal of Urology

      Volume: 22 Issue: 10 Pages: 978-80

    • DOI

      10.1111/iju.12860

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Laparoscopic modified bypass pyeloplasty: a simple procedure for straightforward ureteral spatulation and intracorporeal suturing2015

    • Author(s)
      Haga N, Sato Y, Ogawa S, Yabe M, Akaihata H, Hata J, Ishibashi K, Mizuno K, Hayashi Y, Kojima Y
    • Journal Title

      International Urology and Nephrology

      Volume: 47 Issue: 12 Pages: 1933-1938

    • DOI

      10.1007/s11255-015-1123-9

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression profiling of microRNAs in cryptorchid testes : miR-135a contributes to the maintenance of spermatogonial stem cells by regulating FoxO12014

    • Author(s)
      Moritoki Y, Hayashi Y, Mizuno K, Kamisawa H, Nishio H, Kurokawa S, Ugawa S, Kojima Y, Kohri K
    • Journal Title

      J Urol

      Volume: 191 Issue: 4 Pages: 1174-80

    • DOI

      10.1016/j.juro.2013.10.137

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression analysis of the pluripotency marker UTF-1 for determining the applicability of testis-sparing surgery for prepubertal testis tumors.2013

    • Author(s)
      Mizuno K, Hayashi Y, Kamisawa H, Nishio H, Moritoki Y, Kohri K.
    • Journal Title

      Journal of Pediatric Surgery Case Report

      Volume: 1 Pages: 125-128

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene expression profile during testicular development in patients with SRY-negative 46, XX testicular disorder of sex development2013

    • Author(s)
      Mizuno K, Kojima Y, Kamisawa H, Moritoki Y, Nishio H, Kohri K, Hayashi Y
    • Journal Title

      UROLOGY

      Volume: 82: 1453 Issue: 6 Pages: 1453-1453

    • DOI

      10.1016/j.urology.2013.08.040

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cryptic genomic rearrangements in three patients with 46,XY disorders of sex development2013

    • Author(s)
      M. Igarashi, V. C. Dung, E. Suzuki, S. Ida, M. Nakacho, K. Nakabayashi, K. Mizuno, Y. Hayashi, K. Kohri, Y. Kojima, T. Ogata, M. Fukami
    • Journal Title

      PLoS. One

      Volume: 8 Issue: 7 Pages: e68194-e68194

    • DOI

      10.1371/journal.pone.0068194

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Elucidation of regulatory mechanisms during differentiation of spermatogonial stem cell via androgen action2015

    • Author(s)
      Mizuno K, Hayashi Y, Kamisawa H, Moritoki Y, Nishio H, Kurokawa S, Nakane A, Maruyama T, Yasui T
    • Organizer
      26th European Society of Pediatric Urology Congress
    • Place of Presentation
      プラハ市(チェコ共和国)
    • Year and Date
      2015-10-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Feasibility of laparoscopic orchiopexy for intracanalicular testes in comparative study of surgical outcome with open transinguinal orchiopexy2015

    • Author(s)
      Mizuno K, Hayashi Y, Kamisawa H, Moritoki Y, Nishio H, Kurokawa S, Nakane A, Maruyama T, Yasui T
    • Organizer
      26th European Society of Pediatric Urology Congress
    • Place of Presentation
      プラハ市(チェコ共和国)
    • Year and Date
      2015-10-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 停留精巣が男性不妊症に至る機序とは~閉塞性無精子症患者の精巣検体を用いた研究~2015

    • Author(s)
      神沢 英幸、水野 健太郎、西尾 英紀、守時 良演、岩月 正一郎、黒川 覚史、梅本 幸裕、佐々木 昌一、林 祐太郎、安井 孝周
    • Organizer
      第34回日本アンドロロジー学会学術大会
    • Place of Presentation
      福岡大学病院(福岡県福岡市)
    • Year and Date
      2015-06-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒストンタンパク脱メチル酵素KDM5Aによるヒト停留精巣での造精機能障害機序の解明2015

    • Author(s)
      西尾 英紀、水野 健太郎、神沢 英幸、守時 良演、最上 徹、岩月 正一郎、梅本 幸裕、佐々木 昌一、林 祐太郎、安井 孝周
    • Organizer
      第34回日本アンドロロジー学会学術大会
    • Place of Presentation
      福岡大学病院(福岡県福岡市)
    • Year and Date
      2015-06-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒストンタンパク脱メチル化酵素KDM5Aのヒト造精機能障害への関与2015

    • Author(s)
      西尾 英紀、水野 健太郎、神沢 英幸、守時 良演、最上 徹、林 祐太郎、郡 健二郎
    • Organizer
      第103回日本泌尿器科学会総会
    • Place of Presentation
      石川県立音楽堂、ANAクラウンプラザホテル金沢 他(石川県金沢市)
    • Year and Date
      2015-04-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 精巣発生における細胞接着制御Rhoシグナル伝達系の機能解析2013

    • Author(s)
      水野健太郎、神沢英幸、守時良演、西尾英紀、林祐太郎、郡健二郎
    • Organizer
      第101回日本泌尿器科学会総会
    • Place of Presentation
      さっぽろ芸術文化の館(札幌)
    • Related Report
      2013 Research-status Report
  • [Presentation] 骨盤内異形成腎と腹腔内精巣を合併した一例 ~発症要因の組織学的考察~2013

    • Author(s)
      水野健太郎、西尾英紀、林祐太郎、郡健二郎
    • Organizer
      第22回 日本小児泌尿器科学会総会
    • Place of Presentation
      東京ビッグサイトTFTホール(東京)
    • Related Report
      2013 Research-status Report
  • [Presentation] 精子幹細胞分化におけるアンドロゲンの役割2013

    • Author(s)
      水野健太郎、神沢英幸、守時良演、西尾英紀、岩月正一郎、梅本幸裕、佐々木昌一、林祐太郎、郡健二郎
    • Organizer
      日本アンドロロジー学会第32回学術大会
    • Place of Presentation
      グランキューブ大阪(大阪)
    • Related Report
      2013 Research-status Report
  • [Presentation] ゲノムワイド解析を用いた精巣発生メカニズムの解明2013

    • Author(s)
      水野健太郎、神沢英幸、守時良演、西尾英紀、林祐太郎、郡健二郎
    • Organizer
      第64回名古屋市立大学医学会総会
    • Place of Presentation
      名古屋市立大学(名古屋)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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