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Effective compounds for male infertility patients with reference data for a noninvasive genetic diagnosis

Research Project

Project/Area Number 25462544
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionNagasaki International University

Principal Investigator

Tanaka Hiromitsu  長崎国際大学, 薬学部, 准教授 (10263310)

Co-Investigator(Renkei-kenkyūsha) TSUJIMURA AKIRA  大阪大学, 大学院医学部, 准教授 (40294053)
MIYAGAWA YASUSHI  大阪大学, 大学院医学部, 准教授 (70362704)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsアンドロロジー / 男性不妊症 / 精子形成 / 精巣 / 人工授精 / 遺伝子 / 生薬 / 生殖細胞 / 一塩基多型 / 精子 / 受精 / 不妊症 / 甘草 / Licorice / Isoliquurutingenin / Herbal medicine / Flavonoid / Sperm / Fertilization / Egg / IVF
Outline of Final Research Achievements

Our diagnostic approach enabled us to identify genetic factors in ≥10% of male infertility patients. Furthermore, we found that licorice, which is used as an herbal medicine, increased fertility in mice. In this study, we screened novel chemical substances for in vitro fertilization (IVF) effects using an animal model of male infertility with a specific haploid germ cell gene deficiency. We also analyzed the mechanism underlying the effect of licorice. As a result, we identified a novel peptide that restored fertilization by IVF in male infertile mice (manuscript in preparation). Isoliquiritigenin and formononetin were isolated from licorice as active compounds for IVF. These compounds did not introduce abnormalities in embryogenesis, and both were effective in embryogenesis after IVF in a cow. Our findings demonstrate that effective compounds can be offered to male infertility patients based on the results of a noninvasive genetic diagnosis.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013 2012 Other

All Int'l Joint Research (1 results) Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 7 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Int'l Joint Research] State University of Campinas (UNICAMP)(Brazil)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] SNPs within the Intron-less TAF7 Gene Encoding a General Transcription Factor in Japanese Males.2016

    • Author(s)
      Tanaka H, Yoshimoto T, Nakamura N, Takeda T, Wada M, Miyagwa Y, Akira Tsujimura A.
    • Journal Title

      Nagasaki Int Univ Rev

      Volume: 16

    • NAID

      120005772196

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic Variation in the Testis-Specific Poly(A) Polymerase Beta (PAPOLB) Gene Among Japanese Males.2015

    • Author(s)
      Tanaka H, Tsujimura A, Miyagawa Y, Wada M.
    • Journal Title

      The Open Reprod Sci J

      Volume: 7 Issue: 1 Pages: 1-4

    • DOI

      10.2174/1874255601507010001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] TALEN-Mediated Gene Disruption on Y Chromosome Reveals Critical Role of EIF2S3Y in Mouse Spermatogenesis2015

    • Author(s)
      Matsubara Yohei, Kato Tomoko, Kashimada Kenichi, Tanaka Hiromitsu, Zhi Zhou, Ichinose Shizuko, Mizutani Shuki, Morio Tomohiro, Chiba Tomoki, Ito Yoshiaki, Saga Yumiko, Takada Shuji, and Asahara Hiroshi
    • Journal Title

      Stem Cells and Development

      Volume: 24 Issue: 10 Pages: 1164-1170

    • DOI

      10.1089/scd.2014.0466

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Discovery of a TEKTIN-t/TEKT2 Gene Variant Encoding Sperm Flagellar Protein in Japanese Males.2015

    • Author(s)
      Tanaka H, Tsujimura A, Miyagawa Y, Wada M.
    • Journal Title

      Nagasaki Int Univ Rev

      Volume: 15 Pages: 167-173

    • NAID

      120005625226

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel transcriptional factor Nkapl is a germ cell-specific suppressor of Notch signaling and is indispensable for spermatogenesis.2015

    • Author(s)
      Okuda H, Kiuchi H, Takao T, Miyagawa Y, Tsujimura A, Nonomura N, Miyata H, Okabe M, Ikawa M, Kawakami K, Goshima N, Wada M, Tanaka H.
    • Journal Title

      PLoS One

      Volume: 10

    • NAID

      120006957411

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two activators of in vitro fertilization in mice from licorice.2015

    • Author(s)
      Tung HN, Shoyama Y, Wada M, Tanaka H.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 467 Pages: 447-450

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nucleotide Polymorphism Analysis of Testis-specific CETN1 in Human Male Infertility.2013

    • Author(s)
      Tanaka,H, Tsujimura A, Miyagawa Y, Kohama Y, Aramaki Y, Araki Y, Wada M, Nishimune Y.
    • Journal Title

      The Open Androl J

      Volume: 5 Pages: 1-5

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Standardized Diagram Improves Diagnostic Accuracy and Variability in the Identification of Testicular Cells Prepared for Testicular Sperm Extraction and Intracytoplasmic Sperm Injection.2013

    • Author(s)
      Okuda H, Tsujimura A, Kiuchi H, Matsuoka Y, Takao T, Takada S, Miyagawa Y, Tanaka H, Okamoto Y, Nonomura N.
    • Journal Title

      Urology

      Volume: 82(3) Issue: 3 Pages: 594-594

    • DOI

      10.1016/j.urology.2013.05.023

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Single Nucleotide Polymorphism within the Novel Sex-Linked Testis-Specific Retrotransposed PGAM4 Gene Influences Human Male Fertility2012

    • Author(s)
      Okuda H, 他10名
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 5 Pages: e35195-e35195

    • DOI

      10.1371/journal.pone.0035195

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 精子細胞特異的遺伝子の多型解析とIVFに効果のある生薬成分の臨床応用2016

    • Author(s)
      田中宏光
    • Organizer
      第21回関東アンドロロジーカンファレンス 特別公演
    • Place of Presentation
      東京
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Male infertility and single nucleotide polymorphisms of the testis-specific succinyl CoA:3- oxoacid CoA transferase (SCOT-T/OXCT2) gene in Japanese2016

    • Author(s)
      田中宏光
    • Organizer
      7th International Sciences Conference on Applied Sciences and Engineering
    • Place of Presentation
      Dubai
    • Year and Date
      2016-02-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 精子細胞特異的遺伝子のSNPディスカバリーと臨床応用2015

    • Author(s)
      田中宏光
    • Organizer
      アンドロロジー学会第34回学術大会 共催第22回 精子形成・精巣毒性研究会
    • Place of Presentation
      福岡
    • Year and Date
      2015-06-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 甘草水溶性抽出物の体外成熟、発生培養液への添加が分割率や胚盤胞発生成績に及ぼす影響2014

    • Author(s)
      平田統一
    • Organizer
      日本畜産学会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 男性不妊症遺伝子診断と治療薬の開発2013

    • Author(s)
      田中宏光
    • Organizer
      BIOtech2013
    • Place of Presentation
      東京ビックサイト
    • Related Report
      2013 Research-status Report
  • [Presentation] 甘草抽出物Licoriceのウシ精子処理液あるいは体外受精液への添加が分割率や胚発生成績に及ぼす影響2013

    • Author(s)
      平田統一
    • Organizer
      日本繁殖生物学会
    • Place of Presentation
      東京農工大学農学部府中キャンパス
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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