• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The effects of dioxin on development of endometriosis

Research Project

Project/Area Number 25462560
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionYamaguchi University

Principal Investigator

YAMAGATA Yoshiaki  山口大学, 医学部附属病院, 准教授 (30363120)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords子宮内膜症 / DNAメチル化
Outline of Final Research Achievements

To reveal the mechanism of endometriosis development, the effect of dioxin on cultured eutopic endometrial stromal cells was first investigated. However, no significant effect was recognized by genome wide DNA methylation analysis. During the analysis we found that methyrome rather than transcriptome illustrate individual cell portraits clearly. To clarify the origins of endometriotic lesions, cluster analysis using methyrome data was secondarily conducted. Samples of ovarian endometriosis cells, eutopic endometrium, and normal ovarian cortex etc. were obtained. Extracted DNA was analyzed using HumanMethylation 27/450 followed by cluster analysis. The cluster dendrogram indicated endometriosis cells have similar features to ovary not to eutopic endometrium. These findings imply endometriotic cell in ovary derives from normal ovary.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Genome-wide DNA methylation profiling in cultured eutopic and ectopic endometrial stromal cells.2014

    • Author(s)
      Yamagata Y; Nishino K; Takaki E; Sato S; Maekawa R; Nakai A; Sugino N.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 1 Pages: e83612-e83612

    • DOI

      10.1371/journal.pone.0083612

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ゲノムワイドDNAメチル化解析からみた子宮内膜症の由来2015

    • Author(s)
      山縣芳明
    • Organizer
      第9回日本エピジェネティクス研究会
    • Place of Presentation
      東京一ツ橋学術総合センター(東京都千代田区)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] The origin of endometriosis based on genome wide DNA methylation analysis2015

    • Author(s)
      山縣芳明
    • Organizer
      1st Congress of the Society of Endometriosis and Uterine Disorder
    • Place of Presentation
      Paris Marriott Rive Gauche (Paris, FRANCE)
    • Year and Date
      2015-05-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 卵巣子宮内膜症性嚢胞におけるSTRA6とHSD17B2遺伝子mRNA発現とDNAメチル化解析

    • Author(s)
      山縣芳明
    • Organizer
      第65回日本産科婦人科学会
    • Place of Presentation
      ロイトン札幌(札幌市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 子宮内膜症のレチノール酸代謝異常に関する研究

    • Author(s)
      山縣芳明
    • Organizer
      第58回生殖医学会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 卵巣子宮内膜症性嚢胞におけるSTRA6とHSD17B2遺伝子mRNA発現とDNAメチル化解析

    • Author(s)
      山縣芳明
    • Organizer
      第58回日本人類遺伝学会
    • Place of Presentation
      江陽グランドホテル(仙台市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 子宮内膜症発症進展におけるDNAメチル化異常の関与

    • Author(s)
      山縣芳明
    • Organizer
      第35回日本エンドメトリオーシス学会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Genome-wide DNA methylation profiling in cultured eutopic and ectopic endometrial stromal cells.

    • Author(s)
      Yoshiaki Yamagata
    • Organizer
      29th Annual Meeting of the ESHRE
    • Place of Presentation
      ExCeL London (London, UK)
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi