The mechanism of signal for regulating differentiation in human trophoblast stem cells

• Project/Area Number: 25462562
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kyoto University
• Principal Investigator: TAKAO TOMOKA 京都大学, 医学(系)研究科(研究院), 研究員 (40612429)
• Co-Investigator(Kenkyū-buntansha): WAKE NORIO 九州大学, 環境発達医学研究センター, 特任教授 (60091584)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 分化制御 / 胎盤 / 栄養膜幹細胞 / 分化制御シグナル / ヒト栄養膜幹細胞分化

Outline of Final Research Achievements

We analyzed the new gene for regulating the differentiation of human trophoblast lineage using HTR-8/SVneo cells. We isolated the 3 trophoblast lineages including each functions; human trophoblast stem/precursor(hTS) cells, syncytiotrophoblast(ST) and extravillous trophoblast(EVT)from HTR-8/SVneo. DNA microarray analysis showed that IFIT2(interferon induced protein with tetratricopeptide repeats 2) was up-regulated in ST cells or TRIB3（tribbles pseudokinase 3）was up-regulated in EVT cells. We transfected its into hTS cells. Upregulation of each gene expression was increased trophoblast differentiated cell markers such as HLAG or HCGB.

Research Products

• [Journal Article] Regulation of the Mechanism of TWIST1 Transcription by BHLHE40 and BHLHE41 in Cancer Cells. (2015)
  • Author(s): Asanoma K, Liu G, Yamane T, Miyanari Y, Takao T, Yagi H, Ohgami T, Ichinoe A, Sonoda K, Wake N, Kato K
  • Journal Title: Molecular and Cellular Biology Volume: 35 Issue: 24 Pages: 4096-4109
  • DOI: 10.1128/mcb.00678-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The thermosensitive TRPV3 channel contributes to rapid wound healing in oral epithelia (2015)
  • Author(s): Aijima R, Wang B, Takao T, Mihara H, Kashio M, Ohsaki Y, Zhang JQ, Mizuno A, Suzuki M, Yamashita Y, Masuko S, Goto M, Tominaga M, Kido MA
  • Journal Title: The FASEB Journal Volume: 29 Issue: 1 Pages: 182-192
  • DOI: 10.1096/fj.14-251314
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Establishment of a new diagnostic method for hydropic villi by using TSSC3 antibody. (2013)
  • Author(s): Wake N, Takao T, Asanoma K, Kato H
  • Journal Title: J Obstet Gynaecol Res. Volume: 39(7) Issue: 7 Pages: 1230-1235
  • DOI: 10.1111/jog.12100
  • Peer Reviewed
• [Presentation] ヒト子宮内膜症モデル細胞におけるAd4BP/SF-1の新たな役割 (2015)
  • Author(s): 高尾知佳, 梶谷宇
  • Organizer: 第88回日本内分泌学会学術集会
  • Place of Presentation: 東京都ホテルニューオータニ東京
  • Year and Date: 2015-04-23