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The relationship between endometrial stem cells and epithelial-mesenchymal transfer on the development of endometriosis.

Research Project

Project/Area Number 25462574
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKeio University

Principal Investigator

MASUDA HIROTAKA  慶應義塾大学, 医学部, 講師 (80317198)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Tetsuo  慶應義塾大学, 医学部, 准教授 (10209702)
UCHIDA Hiroshi  慶應義塾大学, 医学部, 専任講師 (90286534)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords子宮内膜症 / 子宮内膜幹細胞 / 上皮間葉転換(EMT) / Side Population / W5C5 / Side Population W5C5 / Side Population W5C5
Outline of Final Research Achievements

Flow cytometry analysis revealed that endometrial side population (SP) cells, CD146+PDGFRβ+ cells and W5C5+ cells were different populations, but these populations overlapped partially. The migration activity of endometrial cells and endometrial SP cells was demonstrated, and the activity was suppressed by an epithelial-mesenchymal transfer inhibitor. We are trying to show this inhibitory effect in vivo using our mouse model.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (39 results)

All 2016 2015 2014 2013 Other

All Journal Article (16 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 4 results) Presentation (22 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Successful treatment of atypical cesarean scar defect using endoscopic surgery.2015

    • Author(s)
      Masuda H, Uchida H, Maruyama T, Sato K, Sato S, Tanaka M
    • Journal Title

      BMC Pregnancy Childbirth

      Volume: 15巻 Issue: 1 Pages: 342-342

    • DOI

      10.1186/s12884-015-0730-x

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Endometrial side population cells: potential adult stem/progenitor cells in endometrium.2015

    • Author(s)
      Masuda H, Maruyama T, Gargett C, Miyazaki K, Matsuzaki Y, Okano H, Tanaka M
    • Journal Title

      Biol Reprod

      Volume: 93巻 Issue: 4 Pages: 1-8

    • DOI

      10.1095/biolreprod.115.131490

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Stem cells in endometrial physiology.2015

    • Author(s)
      Gurung S, Deane JA, Masuda H, Maruyama T, Gargett CE
    • Journal Title

      Semin Reprod Med

      Volume: 33巻 Issue: 05 Pages: 326-332

    • DOI

      10.1055/s-0035-1558405

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] CD34 and CD49f double-positive and lineage marker-negative cells isolated from human myometrium exhibit stem cell-like properties involved in pregnancy-induced uterine remodeling.2015

    • Author(s)
      Ono M, Kajitani T, Uchida H, Arase T, Oda H, Uchida S, Ota K, Nagashima T, Masuda H, Miyazaki K, Asada H, Hida N, Mabuchi Y, Morikawa S, Ito M, Bulun SE, Okano H, Matsuzaki Y, Yoshimura Y, Maruyama T
    • Journal Title

      Biol Reprod

      Volume: 93巻 Issue: 2 Pages: 1-9

    • DOI

      10.1095/biolreprod.114.127126

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Serum estradiol level during withdrawal bleeding as a predictive factor for intermittent ovarian function in women with primary ovarian insufficiency2015

    • Author(s)
      Miyazaki K, Miki F, Uchida S, Masuda H, Uchida H, Maruyama T
    • Journal Title

      Endocrine Journal

      Volume: 62 Issue: 1 Pages: 93-99

    • DOI

      10.1507/endocrj.EJ14-0189

    • NAID

      130004694069

    • ISSN
      0918-8959, 1348-4540
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 臍部トロッカー挿入時に胃損傷をきたし,腹腔鏡下にて修復し得た1例.2015

    • Author(s)
      宮崎 薫,佐藤健二,太田邦明,小川誠司,飯野孝太郎,升田博隆,丸山哲夫,末岡 浩,田中 守
    • Journal Title

      産科と婦人科

      Volume: 82巻 Pages: 832-835

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 付属器摘出後より副角に子宮流血症を発症した1例.2015

    • Author(s)
      世良亜沙子,日原華子,升田博隆,三木史恵、宮崎 薫,太田邦明,内田 浩,丸山哲夫,田中 守,青木大輔
    • Journal Title

      東京産婦人科学会会誌

      Volume: 64巻 Pages: 390-393

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 【着床】 胚の子宮内膜への侵入.2015

    • Author(s)
      内田 浩,升田博隆,三木史恵,日原華子,吉政佑之,片倉慧美,丸山哲夫,田中 守
    • Journal Title

      HORMONE FRONTIER IN GYNECOLOGY

      Volume: 22巻 Pages: 49-54

    • Related Report
      2015 Annual Research Report
  • [Journal Article] System amino acid transporter SNAT2 shows subtype-specific affinity for betaine and hyperosmotic inducibility in placental trophoblasts.2014

    • Author(s)
      Nishimura T, Yagi R, Usuda M, Oda K, Yamazaki M, Suda S, Takahashi Y, Okazaki F, Sai Y, Higuchi, K, Maruyama T, Tomi M, Nakashima E
    • Journal Title

      Biochim Biophys Acta.

      Volume: VOL. 1838 Issue: 5 Pages: 1306-12

    • DOI

      10.1016/j.bbamem.2014.01.004

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] ヒト子宮内膜幹細胞研究の軌跡.2014

    • Author(s)
      升田博隆,丸山哲夫,松崎有未,岡野栄之,吉村泰典
    • Journal Title

      産婦人科の実際

      Volume: 63巻 Pages: 86-7

    • Related Report
      2013 Research-status Report
  • [Journal Article] Achievement of pregnancies in women with primary ovarian insufficiency using close monitoring of follicle development: case reports.2013

    • Author(s)
      Maruyama T, Miyazaki K, Uchida H, Uchida S, Masuda H, Yoshimura Y
    • Journal Title

      Endocr J.

      Volume: VOL. 60 Pages: 791-7

    • NAID

      10031184548

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human uterine stem/ progenitor cells : Implications for uterine physiology and pathology2013

    • Author(s)
      Maruyama T, Miyazaki K, Masuda H, Ono M, Uchida H, Yoshimura Y
    • Journal Title

      Placenta

      Volume: 27 Pages: 77-81

    • DOI

      10.1016/j.placenta.2012.12.010

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] ヒト子宮内膜再構成システムを用いたin vivo幹細胞アッセイの開発と内膜幹細胞の同定.2013

    • Author(s)
      宮崎 薫,丸山哲夫,升田博隆,小田英之,内田明花,内田 浩,吉村泰典
    • Journal Title

      日本生殖内分泌雑誌[平成24年度学術奨励賞受賞]

      Volume: 18巻 Pages: 21-5

    • Related Report
      2013 Research-status Report
  • [Journal Article] ヒト子宮内膜幹細胞の分化能を明らかにするin vivo解析システムの開発.2013

    • Author(s)
      宮崎 薫,丸山哲夫,升田博隆,小田英之,内田明花,内田 浩,吉村泰典
    • Journal Title

      日本エンドメトリオーシス会誌

      Volume: 34巻 Pages: 215-9

    • Related Report
      2013 Research-status Report
  • [Journal Article] 目で見る生殖幹細胞.2013

    • Author(s)
      升田博隆,丸山哲夫,吉村泰典
    • Journal Title

      HORMONE EFONTIER IN GYNECOLOGY

      Volume: 20巻 Pages: 6-9

    • Related Report
      2013 Research-status Report
  • [Journal Article] 【生殖と自然免疫】子宮内膜の粘膜免疫機構.2013

    • Author(s)
      荒瀬 透,丸山哲夫,宮崎 薫,柴田璃香,升田博隆,内田 浩,吉村泰典
    • Journal Title

      HORMONE EFONTIER IN GYNECOLOGY

      Volume: 20巻 Pages: 39-44

    • Related Report
      2013 Research-status Report
  • [Presentation] 脱細胞化骨格のトポロジーが再生子宮の組織極性を決める.2016

    • Author(s)
      三木史恵,丸山哲夫,宮崎 薫,升田博隆,内田 浩,田中 守
    • Organizer
      第15回日本再生医療学会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 子宮内膜と子宮内膜幹細胞そして上皮間葉転換.2016

    • Author(s)
      升田博隆,丸山哲夫,宮崎 薫,松崎有未,岡野栄之,田中 守
    • Organizer
      第37回エンドメトリオーシス学会 [カレント リサーチ・レビュー]
    • Place of Presentation
      ホテル日航熊本(熊本県熊本市)
    • Year and Date
      2016-01-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 磁性体を用いた非侵略的リアルタイム解析可能な子宮内膜症腹膜病変モデルの開発.2016

    • Author(s)
      升田博隆,丸山哲夫,小田英之,肥田直子,内田 浩,三木史恵,日原華子,吉政佑之,片倉慧美,田中 守
    • Organizer
      第37回エンドメトリオーシス学会 [日本エンドメトリオーシス学会演題発表賞(基礎部門)]
    • Place of Presentation
      ホテル日航熊本(熊本県熊本市)
    • Year and Date
      2016-01-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 非典型的な帝王切開瘢痕部症候群に対する治療成功例.2015

    • Author(s)
      升田博隆,佐藤健二,中林 章,佐藤 卓,三木史恵,日原華子,各務真紀,内田 浩,丸山哲夫,吉村泰典,田中 守
    • Organizer
      第55回日本産科婦人科内視鏡学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膣中隔合併の完全中隔子宮に対して子宮鏡下手術を行った一例 ― 子宮頸部中隔切断の有用性 ― .2015

    • Author(s)
      安康真由香,佐藤健二,升田博隆,太田邦明,佐藤 卓,内田 浩,浜谷敏生,丸山哲夫,末岡 浩,田中 守
    • Organizer
      第55回日本産科婦人科内視鏡学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Non-invasive and real-time assessment of peritoneal lesions generated from human endometrial cell transplants locally accumulated by magnetic force in immunodeficient mice.2015

    • Author(s)
      Tetsuo Maruyama, Hirotaka Masuda, Naoko Hida, Hideyuki Oda, Kaoru Miyazaki, Miki Fumie, Hanako Hihara, Kuniaki Ota, Hiroshi Uchida, Mamoru Tanaka
    • Organizer
      31th Annual Meeting of the European Society of Human Reproduction and Embryology (ESHRE).
    • Place of Presentation
      Lisbon, Portugal.
    • Year and Date
      2015-06-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Significance of endometrial stem/ progenitor cells in women with endometriosis.2015

    • Author(s)
      Hirotaka Masuda, Tetsuo Maruyama, Mamoru Tanaka, Caroline Gargett
    • Organizer
      International Federation of Fertility Societies / Japan Society for Reproductive Medicine (IFFS / JSRM) International Meeting 2015. [IFFS Award]
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-04-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Decellularized uterine matrix can promote uterine regeneration in vivo without immune rejection upon allotransplantation.2015

    • Author(s)
      Kaoru Miyazaki, Yuki Ueno, Hirotaka Masuda, Hiroshi Uchida, Tetsuo Maruyama
    • Organizer
      International Federation of Fertility Societies / Japan Society for Reproductive Medicine (IFFS / JSRM) International Meeting 2015
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-04-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 単一抗体によるヒト子宮内膜間葉系幹細胞の新規分離法の開発.2015

    • Author(s)
      升田博隆,丸山哲夫,田中 守
    • Organizer
      第88回日本内分泌学会
    • Place of Presentation
      ホテルニューオータニ(東京都千代田区)
    • Year and Date
      2015-04-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 同種異系移植におけるラット子宮脱細胞化マトリックスの免疫原性と再生組織の内分泌学的検討.2015

    • Author(s)
      上野友輝,宮崎 薫,升田博隆,内田 浩,丸山哲夫
    • Organizer
      第88回日本内分泌学会
    • Place of Presentation
      ホテルニューオータニ(東京都千代田区)
    • Year and Date
      2015-04-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Endometrial stem/ progenitor cells in women with endometriosis.2015

    • Author(s)
      Hirotaka Masuda, Tetsuo Maruyama, Mamoru Tanaka, Daisuke Aoki, Caroline Gargett
    • Organizer
      第67回日本産科婦人科学会 International Session [Good Poster Award]
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-04-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脱細胞化と再細胞化によるラット子宮の再生・再建技術の開発.2014

    • Author(s)
      宮崎 薫,丸山哲夫,升田博隆,肥田直子,内田 浩,吉村泰典
    • Organizer
      第13回日本再生医療学会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 希少部位子宮内膜症発生モデルマウスの開発の試み.2014

    • Author(s)
      小田英之,丸山哲夫,山崎彰子,宮崎 薫,荒瀬 透,内田 浩,升田博隆,吉村泰典
    • Organizer
      第35回日本エンドメトリオーシス学会[日本エンドメトリオーシス学会演題発表賞(基礎部門)]
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 子宮内膜症患者と健常者の月経中腹水・月経血・末梢血に存在する子宮内膜幹細胞の比較.2014

    • Author(s)
      升田博隆,Caroline Gargett,丸山哲夫,吉村泰典
    • Organizer
      第35回日本エンドメトリオーシス学会
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 当院10年間における不育症診療に関する報告.2013

    • Author(s)
      内田明花,丸山哲夫,宮崎 薫,各務真紀,升田博隆,吉村泰典
    • Organizer
      第58回日本生殖医学会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Application of decellularzed rat uterus as a three-dimensional scaffold to in vitro and in vivo uterine tissue engineering.2013

    • Author(s)
      Kaoru Miyazaki, Tetsuo Maruyama, Hirotaka Masuda, Naoko Hida, Hiroshi Uchida, Yasunori Yoshimura
    • Organizer
      69th American Society for Reproductive Medicine (ASRM) Annual Meeting.[Society of Rdproductive Biologists and Technologists (SRBT) BASIC SCIENCE AWARD]
    • Place of Presentation
      Boston, USA.
    • Related Report
      2013 Research-status Report
  • [Presentation] 腹腔鏡下子宮筋腫切除術5年後に発症した臍窩感染の1例.2013

    • Author(s)
      升田博隆,古谷正敬,浅田弘法,伊藤嘉佑子,有馬宏和,内田明花,佐藤健二,佐藤 卓,丸山哲夫,吉村泰典
    • Organizer
      第53回日本産科婦人科内視鏡学会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 慢性甲状腺炎を合併した抗リン脂質抗体強陽性妊娠の一例.2013

    • Author(s)
      丸山哲夫,宮崎 薫,升田博隆,内田 浩,峰岸一宏,松本 直,宮越 敬,吉村泰典
    • Organizer
      第49回日本周産期・新生児医学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Novel decellularization and recellularization techniques for reconstruction and regeneration of the uterus in rat.2013

    • Author(s)
      Kaoru Miyazaki, Tetsuo Maruyama, Hirotaka Masuda, Hideyuki Oda, Naoko Hida, Hiroshi Uchida, Yasunori Yoshimura
    • Organizer
      29th Annual Meeting of the European Society of Human Reprodution and Embryology(ESHRE).
    • Place of Presentation
      London, United Kingdom.
    • Related Report
      2013 Research-status Report
  • [Presentation] 脱細胞化と再細胞化で子宮を作る.2013

    • Author(s)
      宮崎 薫,丸山哲夫,升田博隆,内田 浩,青木大輔,吉村泰典
    • Organizer
      第65回日本産科婦人科学会[高得点演題]
    • Place of Presentation
      ロイトン札幌(北海道札幌市)
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト子宮内膜間葉系幹細胞の新規単一マーカーによる分離法の開発.2013

    • Author(s)
      升田博隆,丸山哲夫,宮崎 薫,青木大輔,吉村泰典,Caroline Gargett
    • Organizer
      第65回日本産科婦人科学会[高得点演題]
    • Place of Presentation
      ロイトン札幌(北海道札幌市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 脱細胞化マトリックスを用いた子宮の再構築.2013

    • Author(s)
      宮崎 薫,丸山哲夫,升田博隆,内田 浩,吉村泰典
    • Organizer
      第86回日本内分泌学会[若手研究奨励賞(YIA)]
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Related Report
      2013 Research-status Report
  • [Remarks] 慶應義塾大学医学部 産婦人科学教室ホームページ内 生殖内分泌研究室

    • URL

      http://www.obgy.med.keio.ac.jp/04research/03rep-endo.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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