A basic and clinical approach to risk factors of recurrent pregnancy loss – Identification of a novel antibody associated with seronegative obstetric antiphospholipid syndrome (SN-APS)
| Project/Area Number |
25462578
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
桑原 慶充 日本医科大学, 医学部, 准教授 (40373013)
峯 克也 日本医科大学, 医学(系)研究科(研究院), 助手 (60409216)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 不育症 / 抗リン脂質抗体症候群（APS) / 血清学的陰性APS / 補体分子C9 / 抗リン脂質抗体症候群 / 血清学的陰性 / 病態責任分子 / プロテオミクス |
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| Outline of Final Research Achievements |
There is a subpopulation of patients with clinical symptoms of APS, but the lack of serological markers (seronegative APS). We collected peripheral blood from SN-APS outpatients with known thrombotic predisposition (protein S deficiency, protein C deficiency, factor XII deficiency, or presence of anti-phosphatidylethanolamine antibodies). Two-dimensional immunoblotting was performed with pooled control and SN-APS sera as the primary antibody, and complement molecule C9 were identified as reactive spots specific to SN-APS serum. Autoantibody for C9 was detected in 2/14 SN-APS patients with no known thrombotic predisposition, and in 1/12 SN-APS patients with a known thrombotic predisposition other than APS by WB using purified C9 treated with iodoacetamide. The reaction was not seen in control sera (0/4). This study suggests that some SN-APS pathologies relate to autoantibodies that react to specific C9 epitopes expressed under fixed conditions.
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Report
(4 results)
Research Products
(3 results)
