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Generation of high-quality embryonic stem cells by new reprogramming technique and a basic research for regenerative medicine

Research Project

Project/Area Number 25462582
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionNational Institute of Health Sciences (2015-2016)
National Research Institute for Child Health and Development (2013)

Principal Investigator

MIURA TAKUMI  国立医薬品食品衛生研究所, 再生・細胞医療製品部, 室長 (60405355)

Co-Investigator(Renkei-kenkyūsha) AKUTSU HIDENORI  国立成育医療研究センター研究所, 生殖医療研究部, 部長 (50347225)
UMEZAWA AKIHIRO  国立成育医療研究センター研究所, 再生医療センター, 再生医療センター長 (70213486)
Research Collaborator SUGAWARA TOHRU  
FUKUDA ATSUSHI  
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
KeywordsiPS細胞 / 幹細胞 / リプログラミング / ES細胞 / 初期化 / 神経幹細胞 / 再生医療
Outline of Final Research Achievements

Several groups have recently reported that naive human embryonic stem cells (hESCs)/human induced pluripotent stem cells (hiPSCs) cultured by the presence of LIF and 2i (two inhibitors) closely resemble the naive pluripotent state of inner cell mass-derived mouse ESCs (mESCs). However, many undefined differences may exist between mESCs and the naive human pluripotent stem cells. In this study, we tried to generate LIF plus 2i-dependent human stem cell-like cells from human fibroblast using iPS-reprogramming factors. As a result, we have developed a method for the conversion of human fibroblasts to LIF-dependent primitive neural stem cells (NSCs) using a strategy based on techniques for the generation of iPSCs. This method may facilitate the generation of patient-specific human neurons for studies and treatment of neurodegenerative diseases.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015 2014 2013

All Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells2017

    • Author(s)
      2.Uchida H, Machida M, Miura T, Kawasaki T, Okazaki T, Sasaki K, Sakamoto S, Ohuchi N, Kasahara M, Umezawa A, Akutsu H.
    • Journal Title

      Journal of Clinical Investigation Insight

      Volume: 2 Issue: 1

    • DOI

      10.1172/jci.insight.86492

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 実験用の細胞培養と臨床用の細胞培養(製造)の違いと考え方2016

    • Author(s)
      三浦巧, 佐藤陽治
    • Journal Title

      細胞培養の基礎知識と細胞培養基材の利用・開発の留意点

      Volume: - Pages: 70-84

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells.2015

    • Author(s)
      2.Akutsu H, Machida M, Kanzaki S, Sugawara T, Ohkura T, Nakamura N, Yamazaki-Inoue M, Miura T, Vemuri MC, Rao MS, Miyado K, Umezawa A.
    • Journal Title

      Regenerative Therapy

      Volume: 1 Pages: 18-29

    • DOI

      10.1016/j.reth.2014.12.004

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Generation of primitive neural stem cells from human fibroblasts using a defined set of factors.2015

    • Author(s)
      3.Miura T, Sugawara T, Fukuda A, Machida M, Tamoto R, Kawasaki T, Umezawa A, Akutsu H.
    • Journal Title

      Biology Open

      Volume: 4 Issue: 11 Pages: 1595-1607

    • DOI

      10.1242/bio.013151

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] ゼノフリー2015

    • Author(s)
      三浦 巧
    • Journal Title

      再生医療

      Volume: 14 Pages: 52-53

    • Related Report
      2015 Research-status Report
  • [Journal Article] Frontier dental research on iPS cells2013

    • Author(s)
      Arakaki M, Egusa H, Otsu K, Saitoh I, Miura T, Harada H.
    • Journal Title

      J Oral Biosci

      Volume: 55 Pages: 191-199

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] β-catenin functions pleiotropically in differentiation and tumorigenesis in mouse embryo-derived stem cells2013

    • Author(s)
      Okumura N, Akutsu H, Sugawara T, Miura T, Takezawa Y, Hosoda A, Yoshida K, Ichida JK, Yamada M, Hamatani T, Kuji N, Miyado K, Yoshimura Y, Umezawa A
    • Journal Title

      PLoS One

      Volume: 8(5) Issue: 5 Pages: 63265-63265

    • DOI

      10.1371/journal.pone.0063265

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Age-associated telomere shortening in mouse oocytes2013

    • Author(s)
      Yamada-Fukunaga T, Yamada M, Hamatani T, Chikazawa N, Ogawa S, Akutsu H, Miura T, Miyado K, Tarin JJ, Kuji N, Umezawa A, Yoshimura Y
    • Journal Title

      Reprod Biol Endocrinol

      Volume: 11 Issue: 1 Pages: 108-108

    • DOI

      10.1186/1477-7827-11-108

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Assessment of Genetic Instability in Human Induced Pluripotent Stem Cells during Long-Term Cell Culture2016

    • Author(s)
      Miura T, Okamura K, Yasuda S, Umezawa A, Sato Y.
    • Organizer
      International Society for Stem Cell Research 2016
    • Place of Presentation
      サンフランシスコ
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 1.The Laws and Regulations for Regenerative Medicine and Cell-Based Therapy in Japan.2015

    • Author(s)
      Takumi Miura
    • Organizer
      World Stem Cell Summit 2015
    • Place of Presentation
      アメリカ合衆国アトランタ
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Generation of Pre-hiPS Cells with Properties Like Neural Progenitors2014

    • Author(s)
      三浦 巧
    • Organizer
      第7回武田科学振興財団薬科学シンポジウム/CiRA国際シンポジウム
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
  • [Presentation] The Combination of Inhibitors of GSK3β and FGF/MEK/ERK Signaling Results In Partially Reprogramming In Human Fibroblasts2013

    • Author(s)
      三浦 巧
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] iPS前駆細胞及びiPS細胞2013

    • Inventor(s)
      三浦 巧、阿久津英憲、梅澤明弘
    • Industrial Property Rights Holder
      ヒューマンサイエンス振興財団
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-082731
    • Filing Date
      2013-04-11
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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