Generation of high-quality embryonic stem cells by new reprogramming technique and a basic research for regenerative medicine
| Project/Area Number |
25462582
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | National Institute of Health Sciences (2015-2016)
National Research Institute for Child Health and Development (2013) |
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Principal Investigator |
MIURA TAKUMI 国立医薬品食品衛生研究所, 再生・細胞医療製品部, 室長 (60405355)
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| Co-Investigator(Renkei-kenkyūsha) |
AKUTSU HIDENORI 国立成育医療研究センター研究所, 生殖医療研究部, 部長 (50347225)
UMEZAWA AKIHIRO 国立成育医療研究センター研究所, 再生医療センター, 再生医療センター長 (70213486)
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| Research Collaborator |
SUGAWARA TOHRU
FUKUDA ATSUSHI
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | iPS細胞 / 幹細胞 / リプログラミング / ES細胞 / 初期化 / 神経幹細胞 / 再生医療 |
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| Outline of Final Research Achievements |
Several groups have recently reported that naive human embryonic stem cells (hESCs)/human induced pluripotent stem cells (hiPSCs) cultured by the presence of LIF and 2i (two inhibitors) closely resemble the naive pluripotent state of inner cell mass-derived mouse ESCs (mESCs). However, many undefined differences may exist between mESCs and the naive human pluripotent stem cells. In this study, we tried to generate LIF plus 2i-dependent human stem cell-like cells from human fibroblast using iPS-reprogramming factors. As a result, we have developed a method for the conversion of human fibroblasts to LIF-dependent primitive neural stem cells (NSCs) using a strategy based on techniques for the generation of iPSCs. This method may facilitate the generation of patient-specific human neurons for studies and treatment of neurodegenerative diseases.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells2017
Author(s)
2.Uchida H, Machida M, Miura T, Kawasaki T, Okazaki T, Sasaki K, Sakamoto S, Ohuchi N, Kasahara M, Umezawa A, Akutsu H.
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Journal Title
Journal of Clinical Investigation Insight
Volume: 2
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells.2015
Author(s)
2.Akutsu H, Machida M, Kanzaki S, Sugawara T, Ohkura T, Nakamura N, Yamazaki-Inoue M, Miura T, Vemuri MC, Rao MS, Miyado K, Umezawa A.
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Journal Title
Regenerative Therapy
Volume: 1
Pages: 18-29
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] β-catenin functions pleiotropically in differentiation and tumorigenesis in mouse embryo-derived stem cells2013
Author(s)
Okumura N, Akutsu H, Sugawara T, Miura T, Takezawa Y, Hosoda A, Yoshida K, Ichida JK, Yamada M, Hamatani T, Kuji N, Miyado K, Yoshimura Y, Umezawa A
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Journal Title
PLoS One
Volume: 8(5)
Issue: 5
Pages: 63265-63265
DOI
Related Report
Peer Reviewed
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[Journal Article] Age-associated telomere shortening in mouse oocytes2013
Author(s)
Yamada-Fukunaga T, Yamada M, Hamatani T, Chikazawa N, Ogawa S, Akutsu H, Miura T, Miyado K, Tarin JJ, Kuji N, Umezawa A, Yoshimura Y
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Journal Title
Reprod Biol Endocrinol
Volume: 11
Issue: 1
Pages: 108-108
DOI
Related Report
Peer Reviewed
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